Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers

被引:42
作者
Hawkes, JS
Bryan, DL
Gibson, RA [1 ]
机构
[1] Flinders Med Ctr, Child Nutr Res Ctr, Bedford Pk, SA 5042, Australia
[2] Flinders Univ S Australia, Dept Paediat & Child Hlth, Bedford Pk, SA 5042, Australia
[3] Child Hlth Res Inst, Adelaide, SA, Australia
关键词
human milk; human milk cells; peripheral blood mononuclear cells; interleukin-1; beta; interleukin-6; tumor necrosis factor-alpha;
D O I
10.1023/A:1020652215048
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Samples of milk (n = 80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40-50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in the mammary gland and have the capacity to respond to further stimulation after leaving the breast.
引用
收藏
页码:338 / 344
页数:7
相关论文
共 30 条
[1]   NORMAL BREAST EPITHELIAL-CELLS PRODUCE INTERLEUKIN-6 AND INTERLEUKIN-8 TOGETHER WITH TUMOR-NECROSIS-FACTOR - DEFECTIVE IL6 EXPRESSION IN MAMMARY-CARCINOMA [J].
BASOLO, F ;
CONALDI, PG ;
FIORE, L ;
CALVO, S ;
TONIOLO, A .
INTERNATIONAL JOURNAL OF CANCER, 1993, 55 (06) :926-930
[2]   HUMAN BREAST-MILK LYMPHOCYTES-T DISPLAY THE PHENOTYPE AND FUNCTIONAL-CHARACTERISTICS OF MEMORY T-CELLS [J].
BERTOTTO, A ;
GERLI, R ;
FABIETTI, G ;
CRUPI, S ;
ARCANGELI, C ;
SCALISE, F ;
VACCARO, R .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1990, 20 (08) :1877-1880
[3]  
CRAGO SS, 1979, CLIN EXP IMMUNOL, V38, P585
[4]   THE IMMUNE-SYSTEM OF HUMAN-MILK - ANTIMICROBIAL, ANTIINFLAMMATORY AND IMMUNOMODULATING PROPERTIES [J].
GOLDMAN, AS .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1993, 12 (08) :664-671
[5]  
Goldman AS, 1997, INT J PEDIAT HEM ONC, V4, P491
[6]   Human milk and host defence:: immediate and long-term effects [J].
Hanson, LÅ .
ACTA PAEDIATRICA, 1999, 88 :42-46
[7]   A randomized trial of supplementation with docosahexaenoic acid-rich tuna oil and its effects on the human milk cytokines interleukin 1β, interleukin 6, and tumor necrosis factor α1-3 [J].
Hawkes, JS ;
Bryan, DL ;
Makrides, M ;
Neumann, MA ;
Gibson, RA .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 2002, 75 (04) :754-760
[8]   Cytokines (IL-1β, IL-6, TNF-α, TGF-β1, and TGF-β2) and prostaglandin E2 in human milk during the first three months postpartum [J].
Hawkes, JS ;
Bryan, DL ;
James, MJ ;
Gibson, RA .
PEDIATRIC RESEARCH, 1999, 46 (02) :194-199
[9]   INVIVO DISTRIBUTION OF HUMAN-MILK LEUKOCYTES AFTER INGESTION BY NEWBORN BABOONS [J].
JAIN, L ;
VIDYASAGAR, D ;
XANTHOU, M ;
GHAI, V ;
SHIMADA, S ;
BLEND, M .
ARCHIVES OF DISEASE IN CHILDHOOD-FETAL AND NEONATAL EDITION, 1989, 64 (07) :930-933
[10]  
Järvinen KM, 2000, CLIN EXP ALLERGY, V30, P637, DOI 10.1046/j.1365-2222.2000.00841.x