Evidence for a role of vertebrate Disp1 in long-range Shh signaling

被引：50

作者：

Etheridge, L. Alton ^{[1
]}

Crawford, T. Quinn ^{[1
]}

Zhang, Shile ^{[2
]}

Roelink, Henk ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Univ Washington, Dept Biol Struct, Sch Med, Seattle, WA 98195 USA

来源：

DEVELOPMENT | 2010年 / 137卷 / 01期

关键词：

Dispatched; 1; Patched; Sonic hedgehog; Polarized epithelium; Morphogen; Neuralized embryoid bodies; Embryonic stem cells; Co-culture; STEROL-SENSING DOMAIN; EMBRYONIC STEM-CELLS; SONIC-HEDGEHOG; PROTEIN; PALMITOYLATION; DIFFERENTIATION; TRAFFICKING; TRANSPORTER; EXOSOMES; POLARITY;

D O I：

10.1242/dev.043547

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dispatched 1 (Disp1) encodes a twelve transmembrane domain protein that is required for long-range sonic hedgehog (Shh) signaling. Inhibition of Disp1 function, both by RNAi or dominant-negative constructs, prevents secretion and results in the accumulation of Shh in source cells. Measuring the Shh response in neuralized embryoid bodies (EBs) derived from embryonic stem (ES) cells, with or without Disp1 function, demonstrates an additional role for Disp1 in cells transporting Shh. Co-cultures with Shh-expressing cells revealed a significant reduction in the range of the contact-dependent Shh response in Disp1(-/-) neuralized EBs. These observations support a dual role for Disp1, not only in the secretion of Shh from the source cells, but also in the subsequent transport of Shh through tissue.

引用

页码：133 / 140

页数：8

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