Overexpression of TaHSF3 in Transgenic Arabidopsis Enhances Tolerance to Extreme Temperatures

被引:74
作者
Zhang, Shuangxi [1 ,2 ,3 ]
Xu, Zhao-Shi [2 ]
Li, Pansong [1 ]
Yang, Le [2 ]
Wei, Yiqin [3 ]
Chen, Ming [2 ]
Li, Liancheng [2 ]
Zhang, Gaisheng [1 ]
Ma, Youzhi [2 ]
机构
[1] Northwest A&F Univ, Coll Agr, Yangling 712100, Shaanxi, Peoples R China
[2] Chinese Acad Agr Sci CAAS, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Inst Crop Sci,Minist Agr, Beijing 100081, Peoples R China
[3] Ningxia Acad Agr Sci, Inst Crop Sci, Ningxia 750105, Peoples R China
基金
中国国家自然科学基金;
关键词
Expression pattern; Heat shock transcription factor; Subcellular localization; Extreme temperature tolerance; Wheat; Arabidopsis; STRESS TRANSCRIPTION FACTORS; HEAT-SHOCK FACTORS; MOLECULAR CHAPERONES; FACTOR FAMILY; RESPONSIVE GENES; OVER-EXPRESSION; PROTEIN-KINASES; IDENTIFICATION; HSFA1; REGULATOR;
D O I
10.1007/s11105-012-0546-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Heat shock factors (HSFs) in plants regulate heat stress response by mediating expression of a set of heat shock protein (HSP) genes. In the present study, we isolated a novel heat shock gene, TaHSF3, encoding a protein of 315 amino acids in wheat. Phylogenetic analysis showed that TaHSF3 belonged to HSF class B2. Subcellular localization analysis indicated that TaHSF3 localized in nuclei. TaHSF3 was highly expressed in wheat spikes and showed intermediate expression levels in roots, stems, and leaves under normal conditions. It was highly upregulated in wheat seedlings by heat and cold and to a lesser extent by drought and NaCl and ABA treatments. Overexpression of TaHSF3 in Arabidopsis enhanced tolerance to extreme temperatures. Frequency of survival of three TaHSF3 transgenic Arabidopsis lines was 75-91 % after heat treatment and 85-95 % after freezing treatment compared to 25 and 10 %, respectively, in wild-type plants (WT). Leaf chlorophyll contents of the transformants were higher (0.52-0.67 mg/g) than WT (0.35 mg/g) after heat treatment, and the relative electrical conductivities of the transformants after freezing treatment were lower (from 17.56 to 18.6 %) than those of WT (37.5 %). The TaHSF3 gene from wheat therefore confers tolerance to extreme temperatures in transgenic Arabidopsis by activating HSPs, such as HSP70.
引用
收藏
页码:688 / 697
页数:10
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