Copy Number Variation of Multiple Genes at Rhg1 Mediates Nematode Resistance in Soybean

被引：490

作者：

Cook, David E. ^{[1
]}

Lee, Tong Geon ^{[2
]}

Guo, Xiaoli ^{[1
]}

Melito, Sara ^{[1
]}

Wang, Kai ^{[3
]}

Bayless, Adam M. ^{[1
]}

Wang, Jianping ^{[2
]}

Hughes, Teresa J. ^{[1
]}

Willis, David K. ^{[4
,5
]}

Clemente, Thomas E. ^{[6
]}

Diers, Brian W. ^{[2
]}

Jiang, Jiming ^{[3
]}

Hudson, Matthew E. ^{[2
,7
]}

Bent, Andrew F. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA

[2] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA

[3] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA

[4] Univ Wisconsin, USDA ARS, Madison, WI 53706 USA

[5] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA

[6] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68588 USA

[7] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

来源：

SCIENCE | 2012年 / 338卷 / 6111期

基金：

美国国家科学基金会;

关键词：

PLANT-PATHOGEN; EXPRESSION; CLUSTERS; ROOTS;

D O I：

10.1126/science.1228746

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The rhg1-b allele of soybean is widely used for resistance against soybean cyst nematode (SCN), the most economically damaging pathogen of soybeans in the United States. Gene silencing showed that genes in a 31-kilobase segment at rhg1-b, encoding an amino acid transporter, an a-SNAP protein, and a WI12 (wound-inducible domain) protein, each contribute to resistance. There is one copy of the 31-kilobase segment per haploid genome in susceptible varieties, but 10 tandem copies are present in an rhg1-b haplotype. Overexpression of the individual genes in roots was ineffective, but overexpression of the genes together conferred enhanced SCN resistance. Hence, SCN resistance mediated by the soybean quantitative trait locus Rhg1 is conferred by copy number variation that increases the expression of a set of dissimilar genes in a repeated multigene segment.

引用

页码：1206 / 1209

页数：4

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