Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus

被引:342
作者
Zhang, JF
Shen, H
Wang, XR [1 ]
Wu, JC
Xue, YQ
机构
[1] Nanjing Univ, State Key Lab Pollut Control & Resoures Reuse, Sch Environm, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Dept Earth Sci, Nanjing 210093, Peoples R China
基金
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词
2,4-dichlorophenol; fish; antioxidant defenses; oxidative stress; long-term exposure;
D O I
10.1016/j.chemosphere.2003.10.048
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
There were few reports on the antioxidant response of aquatic organisms exposed to 2,4-dichlorophenol (2,4-DCP). This research explored the hepatic antioxidant responses of fish to long-term exposure of 2,4-DCP for the first time. Freshwater fish Carassius auratus were chosen as experimental animals. The fish were exposed to six different concentrations of 2,4-DCP (0.005-1.0 mg/l) for 40 days and then liver tissues were separated for determination. As shown from the results, 40 days afterwards, the activities of catalase (CAT) and selenium-dependent glutathione peroxidase (Se-GPx) and the content of oxidized glutathione (GSSG) were induced significantly on the whole compared to control group; superoxide dismutase (SOD) responded to 2,4-DCP exposure at only 0.005 mg/l; the content of reduced glutathione (GSH) was suppressed continuously except Group 7; the activity of glutathione reductase was inhibited initially and then restored to control level from Group 4 on; glutathione S-transferase had only slight responses in Groups 3 and 4. Total glutathione (tGSH) and GSH/GSSG ratio were also calculated to analyze the occurrence of oxidative stress. Besides, good dose-effect relations, which cover most of the exposure concentration range, were found between 2,4-DCP level and CAT activity, GSSG content, Se-GPx activity, respectively. In conclusion, SOD and Se-GPx may be potential early biomarkers of 2,4-DCP contamination in aquatic ecosystems, and further studies will be necessary. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:167 / 174
页数:8
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