In situ detection of human monocyte macrophage serine esterase-1 mRNA expression in human tissues

被引：15

作者：

Satoh, T ^{[1
]}

Hemmerlein, B ^{[1
]}

Zschunke, F ^{[1
]}

Radzun, HJ ^{[1
]}

机构：

[1] Univ Gottingen, Dept Pathol, D-37075 Gottingen, Germany

来源：

PATHOBIOLOGY | 1999年 / 67卷 / 03期

关键词：

human monocyte macrophage serine esterase-1; monocyte macrophage system; in situ hybridization; mRNA;

D O I：

10.1159/000028066

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human monocyte/macrophage serine esterase (HMSE) can be distinguished from esterases of other blood cells by a specific isoelectric focusing pattern of five enzyme variants. Using a HMSE-1 cDNA, expression of mRNA of human biopsy specimens and cytospin preparations. HMSE-1 transcripts could be detected in the myelomonocytic cell line U-937, blood monocytes and in tissue macrophages. Immune accessory cells of the monocyte/macrophage lineage did not express detectable amounts of HMSE-1 mRNA. These results indicate that HMSE-1 represents a cell-lineage-specific enzyme which can be used to characterize physiological and neoplastic variants of the human monocyte/macrophage cell system. The method described will enable further insight into the physiological function of HMSE-1.

引用

页码：158 / 162

页数：5

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