Molecular characterization of laccase genes from the basidiomycete Trametes hirsuta Bm-2 and analysis of the 5 untranslated region (5UTR)

被引：7
作者：
Pereira-Patron, Alejandrina [1 ]
Solis-Pereira, Sara [1 ]
Lizama-Uc, Gabriel [1 ]
Ramirez-Prado, Jorge H. [2 ]
Perez-Brito, Daisy [3 ]
Tapia-Tussell, Raul [4 ]
机构：
[1] Inst Tecnol Merida, Tecnol Nacl Mexico, Dept Ingn Quim & Bioquim, Av Tecnol Km 4-5 S-N, Merida 97118, Yucatan, Mexico
[2] Ctr Invest Cient Yucatan, Unidad Biotecnol, Calle 43 130 X 32 Y 34, Merida 97205, Yucatan, Mexico
[3] Ctr Invest Cient Yucatan, Lab GeMBio, Calle 43 130 X 32 Y 34, Merida 97205, Yucatan, Mexico
[4] Ctr Invest Cient Yucatan, Unidad Energia Renovable, Carretera Sierra Papacal Chuburna Puerto Km 5, Merida 97302, Yucatan, Mexico
来源：
3 BIOTECH | 2019年 / 9卷 / 04期
关键词：
Trametes hirsuta; Laccase genes; RACE-PCR; Untranslated regions; WHITE-ROT BASIDIOMYCETE; MULTIGENE FAMILY; FUNGAL LACCASES; MESSENGER-RNAS; EXPRESSION; SEQUENCE; IDENTIFICATION; TRANSLATION; LANDSCAPE; PROTEINS;
D O I：
10.1007/s13205-019-1691-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this study was to identify and characterize laccase genes produced by Trametes hirsuta Bm-2 in a liquid medium, both with and without induction. The amplification of 5and 3regions of laccase sequences was obtained by the RACE-PCR method, and these were assembled to obtain a cDNA of total length. Two new laccase genes were isolated from basal medium (lac-B) and lignocellulosic grapefruit substrate (lac-T), both encoding open reading frames of 2566bp. Both laccase-predicted proteins consisted of 521 amino acids, four copper-binding regions, a signal peptide, and five potential glycosilation sites (Asn-Xaa-Ser/Tre). Moreover, the deduced amino acid sequences share about 76-85% identity with other laccases of WRF. Sequence comparison showed 47 synonymous point mutations between lac-B and lac-T. In addition, 5 untranslated regions (UTR) of laccase genes lac-B and lac-T showed differences in length and number of regulatory elements that may affect transcriptional or translational expression of these genes.
引用
收藏
页数：12
相关论文
共 49 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Development of novel enzymatic bioremediation process for textile industry effluents through response surface methodology [J].
Asgher, Muhammad ;
Yasmeen, Qamar ;
Iqbal, Hafiz Muhammad Nasir .
ECOLOGICAL ENGINEERING, 2014, 63 :1-11
[3]   Fungal laccases - occurrence and properties [J].
Baldrian, P .
FEMS MICROBIOLOGY REVIEWS, 2006, 30 (02) :215-242
[4]  
Barrett L.W., 2013, UNTRANSLATED GENE RE, P1, DOI DOI 10.1007/978-3-0348-0679-4
[5]   Longer First Introns Are a General Property of Eukaryotic Gene Structure [J].
Bradnam, Keith R. ;
Korf, Ian .
PLOS ONE, 2008, 3 (08)
[6]  
Brijwani K., 2010, ENZYME RES, V2010, DOI 10.4061/2010/149748
[7]   Comprehensive annotation of the transcriptome of the human fungal pathogen Candida albicans using RNA-seq [J].
Bruno, Vincent M. ;
Wang, Zhong ;
Marjani, Sadie L. ;
Euskirchen, Ghia M. ;
Martin, Jeffrey ;
Sherlock, Gavin ;
Snyder, Michael .
GENOME RESEARCH, 2010, 20 (10) :1451-1458
[8]   Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution [J].
Bulter, T ;
Alcalde, M ;
Sieber, V ;
Meinhold, P ;
Schlachtbauer, C ;
Arnold, FH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (02) :987-995
[9]   Efficient cap-dependent translation of mammalian mRNAs with long and highly structured 5'-untranslated regions in vitro and in vivo [J].
Dmitriev, S. E. ;
Andreev, D. E. ;
Adyanova, Z. V. ;
Terenin, I. M. ;
Shatsky, I. N. .
MOLECULAR BIOLOGY, 2009, 43 (01) :108-113
[10]   MUSCLE: multiple sequence alignment with high accuracy and high throughput [J].
Edgar, RC .
NUCLEIC ACIDS RESEARCH, 2004, 32 (05) :1792-1797
12345