Quantitative Genomic and Antigenomic Enterovirus RNA Detection in Explanted Heart Tissue Samples from Patients with End-Stage Idiopathic Dilated Cardiomyopathy

被引：26

作者：

Leveque, Nicolas ^{[1
,2
]}

Renois, Fanny ^{[1
,2
]}

Talmud, Deborah ^{[1
,2
]}

Yohan Nguyen ^{[1
,2
]}

Lesaffre, Francois ^{[2
,3
]}

Boulagnon, Camille ^{[4
]}

Bruneval, Patrick ^{[5
]}

Fornes, Paul ^{[2
,4
]}

Andreoletti, Laurent ^{[1
,2
]}

机构：

[1] Univ Hosp, Clin & Mol Virol Unit, Reims, France

[2] Fac Med, SFR CAP Sante, EA CardioVir 4684, Reims, France

[3] Univ Hosp, Dept Cardiol, Reims, France

[4] Univ Hosp, Dept Pathol, Reims, France

[5] Hop Europeen Georges Pompidou, Dept Pathol, Paris, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 10期

关键词：

VIRAL-RNA; ENDOMYOCARDIAL TISSUE; COXSACKIEVIRUS B3; MYOCARDITIS; REPLICATION; DELETIONS; DISEASE; ASSOCIATION; PERSISTENCE; INFECTION;

D O I：

10.1128/JCM.01612-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Standardized one-step real-time RT-PCR assay detected enterovirus RNA in cardiac biopsy samples from 4 of 20 patients suffering from idiopathic dilated cardiomyopathy (IDCM). The median viral load was 287 copies per microgram of total extracted nucleic acids, with positive-to negative-strand RNA ratios ranging from 2 to 20. These results demonstrate enterovirus persistence in the heart of IDCM patients, characterized by low viral loads and low positive- to negative-RNA ratios.

引用

页码：3378 / 3380

页数：3

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