Quantitative Genomic and Antigenomic Enterovirus RNA Detection in Explanted Heart Tissue Samples from Patients with End-Stage Idiopathic Dilated Cardiomyopathy

被引：26

作者：

Leveque, Nicolas ^{[1
,2
]}

Renois, Fanny ^{[1
,2
]}

Talmud, Deborah ^{[1
,2
]}

Yohan Nguyen ^{[1
,2
]}

Lesaffre, Francois ^{[2
,3
]}

Boulagnon, Camille ^{[4
]}

Bruneval, Patrick ^{[5
]}

Fornes, Paul ^{[2
,4
]}

Andreoletti, Laurent ^{[1
,2
]}

机构：

[1] Univ Hosp, Clin & Mol Virol Unit, Reims, France

[2] Fac Med, SFR CAP Sante, EA CardioVir 4684, Reims, France

[3] Univ Hosp, Dept Cardiol, Reims, France

[4] Univ Hosp, Dept Pathol, Reims, France

[5] Hop Europeen Georges Pompidou, Dept Pathol, Paris, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 10期

关键词：

VIRAL-RNA; ENDOMYOCARDIAL TISSUE; COXSACKIEVIRUS B3; MYOCARDITIS; REPLICATION; DELETIONS; DISEASE; ASSOCIATION; PERSISTENCE; INFECTION;

D O I：

10.1128/JCM.01612-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Standardized one-step real-time RT-PCR assay detected enterovirus RNA in cardiac biopsy samples from 4 of 20 patients suffering from idiopathic dilated cardiomyopathy (IDCM). The median viral load was 287 copies per microgram of total extracted nucleic acids, with positive-to negative-strand RNA ratios ranging from 2 to 20. These results demonstrate enterovirus persistence in the heart of IDCM patients, characterized by low viral loads and low positive- to negative-RNA ratios.

引用

页码：3378 / 3380

页数：3

共 18 条

[1]

Andreoletti L, 1996, J MED VIROL, V48, P53, DOI 10.1002/(SICI)1096-9071(199601)48:1<53::AID-JMV9>3.0.CO

[2]

2-K

[3] Enteroviruses can persist with or without active viral replication in cardiac tissue of patients with end-stage ischemic or dilated cardiomyopathy [J].

Andréoletti, L ;

Bourlet, T ;

Moukassa, D ;

Rey, L ;

Hot, D ;

Li, YW ;

Lambert, V ;

Gosselin, B ;

Mosnier, JF ;

Stankowiak, C ;

Wattré, P .

JOURNAL OF INFECTIOUS DISEASES, 2000, 182 (04) :1222-1227

[4] Active coxsackleviral B infection is associated with disruption of dystrophin in endomyocardial tissue of patients who died suddenly of acute myocardial infarction [J].

Andreoletti, Laurent ;

Venteo, Lydie ;

Douche-Aourik, Fatima ;

Canas, Frederic ;

de la Grandmaison, Geoffroy Lorin ;

Jacques, Jerome ;

Moret, Helene ;

Jovenin, Nicolas ;

Mosnier, Jean-Francois ;

Matta, Mathieu ;

Duband, Sebastien ;

Pluot, Michel ;

Pozzetto, Bruno ;

Bourlet, Thomas .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2007, 50 (23) :2207-2214

[5]

Aretz H T, 1987, Am J Cardiovasc Pathol, V1, P3

[6] Meta-analysis of the association of enteroviruses with human heart disease [J].

Baboonian, C ;

Treasure, T .

HEART, 1997, 78 (06) :539-543

[7] Human Parvovirus B19-Associated Myocarditis. [J].

Bock, Claus-Thomas ;

Klingel, Karin ;

Kandolf, Reinhard .

NEW ENGLAND JOURNAL OF MEDICINE, 2010, 362 (13) :1248-1249

[8] 5' terminal deletions in the genome of a coxsackievirus B2 strain occurred naturally in human heart [J].

Chapman, Nora M. ;

Kim, Kyung-Soo ;

Drescher, Kristen M. ;

Oka, Kuniyuki ;

Tracy, Steven .

VIROLOGY, 2008, 375 (02) :480-491

[9] The role of endomyocardial biopsy in the management of cardiovascular disease - A scientific statement from the American Heart Association, the American College of Cardiology, and the European Society of Cardiology [J].

Cooper, Leslie T. ;

Baughman, Kenneth L. ;

Feldman, Arthur M. ;

Frustaci, Andrea ;

Jessup, Mariell ;

Kuhl, Uwe ;

Levine, Glenn N. ;

Narula, Jagat ;

Starling, Randall C. ;

Towbin, Jeffrey ;

Virmani, Renu .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2007, 50 (19) :1914-1931

[10] Acute viral myocarditis [J].

Dennert, Robert ;

Crijns, Harry J. ;

Heymans, Stephane .

EUROPEAN HEART JOURNAL, 2008, 29 (17) :2073-2082

← 1 2 →