Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation

被引：28

作者：

Antkowiak, Maciej ^{[1
,2
]}

Torres-Mapa, Maria Leilani ^{[1
]}

Dholakia, Kishan ^{[1
]}

Gunn-Moore, Frank J. ^{[2
]}

机构：

[1] Univ St Andrews, Sch Phys & Astron, SUPA, St Andrews KY16 9SS, Fife, Scotland

[2] Univ St Andrews, Sch Biol, SULSA, St Andrews KY16 9TS, Fife, Scotland

来源：

BIOMEDICAL OPTICS EXPRESS | 2010年 / 1卷 / 02期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1364/BOE.1.000414

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viability and photoporation efficiency. With the latter, the results provide a new insight into the efficiency and toxicity of this novel optical method of drug delivery. In addition, quantitative phase maps reveal photoporation related sub-cellular dynamics of cytoplasmic vesicles. (C) 2010 Optical Society of America

引用

页码：414 / 424

页数：11

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