A sensitive and validated HPLC-MS/MS method for simultaneous determination of seven coccidiostats in bovine whole milk

被引：18

作者：

Nebot, Carolina ^{[1
]}

Iglesias, Alejandra ^{[1
]}

Regal, Patricia ^{[1
]}

Manuel Miranda, Jose ^{[1
]}

Fente, Cristina ^{[1
]}

Cepeda, Alberto ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Vet Med, Dept Analyt Chem Nutr & Bromatol, Lugo 27002, Spain

来源：

FOOD CONTROL | 2012年 / 27卷 / 01期

关键词：

Coccidiostats; Milk; High-performance liquid chromatography; Mass spectrometry; 2009/124/EC; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; ENDOCRINE DISRUPTING CHEMICALS; LC-MS-MS; VETERINARY DRUGS; EGGS; QUANTIFICATION; NICARBAZIN; EFFICIENT; MONENSIN;

D O I：

10.1016/j.foodcont.2012.02.012

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Sensitive and reliable methods for the identification and quantification of coccidiostats in milk are needed as the European Parliament had established maximum level for these substances in milk (Commission Regulation 124/2009/EC). This study presents a method for the simultaneous extraction, identification, and quantification of seven coccidiostats (robenidine, decoquinate, monensin, salinomycin, lasalocid, maduramicin, and narasin) in bovine whole milk. The method was validated in-house in accordance with the European Commission Decision 2002/657/EC. Recoveries, decision limit, and limit of quantification values ranged from 69 to 109%, 9.9-18.4 mu g kg(-1), and 0.4-1.0 mu g kg(-1), respectively. The method was successfully applied to a total of 120 samples obtained from dairy farms located in the northwest of Spain, and decoquinate was detected in one of them. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：29 / 36

页数：8

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