Germ line stem cell competition in postnatal mouse testes

被引:69
作者
Shinohara, T [1 ]
Orwig, KE [1 ]
Avarbock, MR [1 ]
Brinster, RL [1 ]
机构
[1] Univ Penn, Sch Vet Med, Dept Anim Biol, Philadelphia, PA 19104 USA
关键词
developmental biology; gamete biology; male reproductive tract; spermatogenesis; testis;
D O I
10.1095/biolreprod66.5.1491
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Niche is believed to affect stem cell behavior. In self-renewing systems for which functional transplantation assays are available, it has long been assumed that stem cells are fixed in the niche and that ablative treatments to remove endogenous stem cells are required for successful donor engraftment. Our results demonstrate that enriched populations of donor stem cells can produce long-lasting spermatogenic colonies in testes of immature and mature, nonablated mice, albeit at a lower frequency than in ablated mice. Colonization of nonablated recipient testes by neonate, pup, and cryptorchid adult donor spermatogonial stem cells demonstrates that competition for niche begins soon after birth and that endogenous stem cells influence the degree and pattern of donor cell colonization. Thus, a dynamic relationship between stem cell and niche exists in the testis, as has been suggested for hematopoiesis. Therefore, similar competitive properties of donor stem cells may be characteristic of all self-renewing systems.
引用
收藏
页码:1491 / 1497
页数:7
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