Caffeic acid induces keratinocyte differentiation by activation of PPAR-α

被引：16

作者：

Kim, Bora ^{[1
]}

Kim, Jin Eun ^{[1
]}

Kim, Hyun-Soo ^{[2
]}

机构：

[1] Enprani Co Ltd, R&D Ctr Skin Sci & Cosmet, Inchon, South Korea

[2] Jungwon Univ, Dept Food Sci & Ind, Chungbuk 367805, South Korea

来源：

JOURNAL OF PHARMACY AND PHARMACOLOGY | 2014年 / 66卷 / 01期

关键词：

caffeic acid; keratinocyte differentiation; peroxisome proliferator-activated receptors; structure-based pharmacophore screening; EPIDERMAL PERMEABILITY BARRIER; PHENETHYL ESTER; MOUSE SKIN; RECEPTORS; EXPRESSION; DERIVATIVES; MICE;

D O I：

10.1111/jphp.12159

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

ObjectivesPeroxisome proliferator-activated receptors (PPAR)- plays an important role in epidermal differentiation and barrier recovery, and topical treatment with PPAR- agonists restores epidermal homeostasis in essential fatty acid deficiency and permeability barrier in skin disruptions. Therefore, we performed structure-based pharmacophore screening to search for a novel PPAR- agonist. Caffeic acid was ultimately selected and evaluated for its effects on keratinocyte differentiation and epidermal permeability barrier. MethodsThe transactivation activity of PPAR-responsive element (PPRE) and cornified envelope (CE) formation were assayed. Also, immunoblot analysis and anti-oxidant activity were investigated on caffeic acid. Key findingsCaffeic acid increases the transactivation activity of PPRE and CE formation in keratinocytes. In addition, caffeic acid promotes the expression of genes and proteins related to CE formation such as involucrin and transglutaminase-1. Additionally, anti-oxidant activity were improved by caffeic acid. ConclusionsCaffeic acid can promote keratinocyte differentiation and restore skin barrier homeostasis and is suggested to be an appropriate skin therapeutic agent for improving epidermal permeability barrier function.

引用

页码：84 / 92

页数：9

共 35 条

[1] Human epidermal keratinocytes undergo (-)-epigallocatechin-3-gallate-dependent differentiation but not apoptosis [J].

Balasubramanian, S ;

Sturniolo, MT ;

Dubyak, GR ;

Eckert, RL .

CARCINOGENESIS, 2005, 26 (06) :1100-1108

[2] Calcium- and vitamin D-regulated keratinocyte differentiation [J].

Bikle, DD ;

Ng, D ;

Tu, CL ;

Oda, Y ;

Xie, Z .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2001, 177 (1-2) :161-171

[3]

DOWNING DT, 1992, J LIPID RES, V33, P301

[4] PPAR-alpha in cutaneous inflammation [J].

Dubrac, Sandrine ;

Schmuth, Matthias .

DERMATO-ENDOCRINOLOGY, 2011, 3 (01) :23-26

[5] Stratum corneum defensive functions: An integrated view [J].

Elias, PM .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2005, 125 (02) :183-200

[6] PREFERENTIAL CYTO-TOXICITY ON TUMOR-CELLS BY CAFFEIC ACID PHENETHYL ESTER ISOLATED FROM PROPOLIS [J].

GRUNBERGER, D ;

BANERJEE, R ;

EISINGER, K ;

OLTZ, EM ;

EFROS, L ;

CALDWELL, M ;

ESTEVEZ, V ;

NAKANISHI, K .

EXPERIENTIA, 1988, 44 (03) :230-232

[7] Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid) [J].

Gülçin, I .

TOXICOLOGY, 2006, 217 (2-3) :213-220

[8] Activators of the nuclear hormone receptors PPAR alpha and FXR accelerate the development of the fetal epidermal permeability barrier [J].

Hanley, K ;

Jiang, Y ;

Crumrine, D ;

Bass, NM ;

Appel, R ;

Elias, PM ;

Williams, ML ;

Feingold, KR .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (03) :705-712

[9] Dietary Glucosylceramide Enhances Cornified Envelope Formation via Transglutaminase Expression and Involucrin Production [J].

Hasegawa, Tatsuya ;

Shimada, Haruo ;

Uchiyama, Taro ;

Ueda, Osamu ;

Nakashima, Masaya ;

Matsuoka, Yasuhiro .

LIPIDS, 2011, 46 (06) :529-535

[10]

HOLLERAN WM, 1994, J LIPID RES, V35, P905

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