Increased NPC1L1 and ACAT2 expression in the jejunal mucosa from Chinese gallstone patients

被引：28

作者：

Jiang, Zhao-Yan ^{[1
,7
]}

Jiang, Chong-Yi ^{[7
]}

Wang, Lei ^{[2
]}

Wang, Jian-Cheng ^{[7
]}

Zhang, Sheng-Dao ^{[7
]}

Einarsson, Curt ^{[3
]}

Eriksson, Mats ^{[4
,5
,6
]}

Han, Tian-Quan ^{[7
]}

Parini, Paolo ^{[1
,6
]}

Eggertsen, Gosta ^{[1
]}

机构：

[1] Karolinska Univ, Div Clin Chem, Dept Lab Med, Huddinge Hosp, SE-14186 Stockholm, Sweden

[2] Shanghai Jiao Tong Univ, Dept Gastroenterol, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China

[3] Karolinska Univ, Dept Gastroenterol, Huddinge Hosp, SE-14186 Stockholm, Sweden

[4] Karolinska Univ, Metab Unit, Dept Diabet Endocrinol & Metab, Huddinge Hosp,Karolinska Inst, SE-14186 Stockholm, Sweden

[5] Karolinska Univ, Dept Med, Karolinska Inst, Huddinge Hosp, SE-14186 Stockholm, Sweden

[6] Novum, Dept Biosci & Nutr, Karolinska Inst, SE-14186 Stockholm, Sweden

[7] Shanghai Jiao Tong Univ, Dept Surg, Shanghai Inst Digest Surg, Ruijin Hosp,Sch Med, Shanghai 200025, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 01期

基金：

瑞典研究理事会; 中国国家自然科学基金;

关键词：

Cholesterol gallstone disease; NPC1L1; ACAT2; ABCG5/ABCG8; Gene expression; Polymorphism; Plant sterol; 7; alpha-hydroxy-4-cholesten-3-one; INTESTINAL CHOLESTEROL ABSORPTION; DIETARY-CHOLESTEROL; BILIARY LIPIDS; PLASMA; EZETIMIBE; DISEASE; TARGET; ABCG8; MICE; BILE;

D O I：

10.1016/j.bbrc.2008.11.131

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The incidence of cholesterol gallstones is a very common disease. The aim of this study is to probe for underlying intestinal molecular defects associated with development of gallstones. Twelve Chinese patients with cholesterol gallstone disease (GS) and 31 gallstone-free (GSF) patients were investigated. Quantitation of mRNA levels for individual genes in mucosal biopsies from jejunum was carried out with real-time PCR. The frequency of two SNPs in the ABCG8 gene (Y54C and T400K) was determined by allelic discrimination. The intestinal mRNA expression of NPC1L1 and ACAT2 were significantly higher in GS than GSF (P < 0.05). No differences were observed concerning the levels for plasma lipids, plant sterols and 7 alpha-hydroxy-4-cholesten-3-one between GS and GSF. No correlations were observed between patients carrying the different genotypes for Y54C or T400K and their mRNA levels for ABCG5 or ABCG8. The increased NPC1L1 and ACAT2 mRNA levels in gallstone patients might indicate an upregulated absorption and esterification of cholesterol in the small intestine. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：49 / 54

页数：6

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