A microRNA expression signature of human solid tumors defines cancer gene targets

被引：4720
作者：
Volinia, S
Calin, GA
Liu, CG
Ambs, S
Cimmino, A
Petrocca, F
Visone, R
Iorio, M
Roldo, C
Ferracin, M
Prueitt, RL
Yanaihara, N
Lanza, G
Scarpa, A
Vecchione, A
Negrini, M
Harris, CC
Croce, CM [1 ]
机构：
[1] Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA
[2] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA
[3] NCI, Canc Res Ctr, Human Carcinogenesis Lab, Bethesda, MD 20892 USA
[4] Univ Ferrara, Telethon Facil Data Min Anal DNA Microarrays, Dept Morphol & Enbryol, I-44100 Ferrara, Italy
[5] Univ Ferrara, Dept Expt & Diagnost Med, I-44100 Ferrara, Italy
[6] Univ Ferrara, Interdept Ctr Canc Res, I-44100 Ferrara, Italy
[7] Univ Verona, Dept Pathol, I-37100 Verona, Italy
[8] St Andrea Hosp, Dept Histopathol, I-00185 Rome, Italy
[9] Univ Roma La Sapienza, I-00185 Rome, Italy
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 07期
关键词：
microarray; transcriptome; tumorigenesis;
D O I：
10.1073/pnas.0510565103
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signature composed by a large portion of overexpressed miRNAs. Among these miRNAs are some with well characterized cancer association, such as miR-17-5p, miR-20a, miR-21, miR-92, miR-106a, and miR-155. The predicted targets for the differentially expressed miRNAs are significantly enriched for protein-coding tumor suppressors and oncogenes (P < 0.0001). A number of the predicted targets, including the tumor suppressors RB1 (Retinoblastoma 1) and TGFBR2 (transforming growth factor, beta receptor II) genes were confirmed experimentally. Our results indicate that miRNAs are extensively involved in cancer pathogenesis of solid tumors and support their function as either dominant or recessive cancer genes.
引用
收藏
页码：2257 / 2261
页数：5
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