Identifying chondrogenesis strategies for tissue engineering of articular cartilage

被引：24

作者：

Chen, Michael J. ^{[1
,2
]}

Whiteley, Jonathan P. ^{[3
]}

Please, Colin P. ^{[2
]}

Ehlicke, Franziska ^{[4
]}

Waters, Sarah L. ^{[2
]}

Byrne, Helen M. ^{[2
]}

机构：

[1] Univ Adelaide, Sch Math Sci, North Terrace, Adelaide, SA 5005, Australia

[2] Univ Oxford, Math Inst, Oxford, England

[3] Univ Oxford, Dept Comp Sci, Oxford, England

[4] Univ Hosp Wurzburg, Dept Tissue Engn & Regenerat Med, Wurzburg, Germany

来源：

JOURNAL OF TISSUE ENGINEERING | 2019年 / 10卷

基金：

英国工程与自然科学研究理事会;

关键词：

Tissue engineering; chondrogenesis; TGF-beta; reaction-diffusion model; LATENT TRANSFORMING GROWTH-FACTOR-BETA-1; TGF-BETA BINDING; EXTRACELLULAR-MATRIX; CHONDROCYTES; RECEPTOR;

D O I：

10.1177/2041731419842431

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

A key step in the tissue engineering of articular cartilage is the chondrogenic differentiation of mesenchymal stem cells (MSCs) into chondrocytes (native cartilage cells). Chondrogenesis is regulated by transforming growth factor-beta (TGF-beta), a short-lived cytokine whose effect is prolonged by storage in the extracellular matrix. Tissue engineering applications aim to maximise the yield of differentiated MSCs. Recent experiments involve seeding a hydrogel construct with a layer of MSCs lying below a layer of chondrocytes, stimulating the seeded cells in the construct from above with exogenous TGF-beta and then culturing it in vitro. To investigate the efficacy of this strategy, we develop a mathematical model to describe the interactions between MSCs, chondrocytes and TGF-beta. Using this model, we investigate the effect of varying the initial concentration of TGF-beta, the initial densities of the MSCs and chondrocytes, and the relative depths of the two layers on the long-time composition of the tissue construct.

引用

页数：14

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