Direct electrocatalytic oxidation of adenine and guanine on carbon ionic liquid electrode and the simultaneous determination

被引：149

作者：

Sun, Wei ^{[1
]}

Li, Yinzhuo ^{[1
]}

Duan, Yuanyuan ^{[1
]}

Jiao, Kui ^{[1
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Key Lab Ecochem Engn, Minist Educ, Qingdao 266042, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 04期

基金：

中国国家自然科学基金;

关键词：

N-Butylpyridinium hexafluorophosphate; Adenine; Guanine; DNA; Carbon ionic liquid electrode;

D O I：

10.1016/j.bios.2008.07.068

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A carbon ionic liquid electrode (CILE) was fabricated by using anionic liquid of N-butylpyridinium hexafluorophosphate (BPPF(6)) as binder and further used for the simultaneous detection of adenine and guanine. The direct electrooxidation behaviors of adenine and guanine were carefully investigated on the CILE. The results indicated that both adenine and guanine showed the increase of the oxidation peak currents with the negative shift of the oxidation peak potentials in contrast to that on the traditional carbon paste electrode (CPE). The electrochemical parameters of adenine and guanine on the CILE were calculated and a new electroanalytical method was established for the detection of adenine and guanine, respectively. The CILE exhibited good behaviors in the simultaneous detection of adenine and guanine with the peak separation as 0.304V. The measurements of thermally denatured single-stranded DNA (ssDNA) were further carried out and the value of (G + C)/(A + T) of ssDNA was calculated as 0.81. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：988 / 993

页数：6

共 37 条

[1] Electrochemical DNA biosensor for environmental monitoring [J].

Chiti, G ;

Marrazza, G ;

Mascini, M .

ANALYTICA CHIMICA ACTA, 2001, 427 (02) :155-164

[2]

Davidson J. N., 1972, BIOCH NUCL ACIDS, P129

[3] Direct electrochemical oxidation of DNA on polycrystalline gold electrodes [J].

Ferapontova, EE ;

Dominguez, E .

ELECTROANALYSIS, 2003, 15 (07) :629-634

[4] Voltammetric determination of adenosine and guanosine using fullerene-C60-modified glassy carbon electrode [J].

Goyal, Rajendra N. ;

Gupta, Vinod K. ;

Oyama, Munetaka ;

Bachheti, Neeta .

TALANTA, 2007, 71 (03) :1110-1117

[5]

HARTWICK RA, 1997, J CHROMATOGR, V143, P383

[6] Electrochemical behavior and detection of guanine using a sodium montmorillonite-modified carbon paste electrode [J].

Huang, W ;

Zhang, S ;

Wu, Y .

RUSSIAN JOURNAL OF ELECTROCHEMISTRY, 2006, 42 (02) :153-156

[7] Voltammetric studies of the interaction of nogalamycin antitumor drug with DNA [J].

Ibrahim, MS .

ANALYTICA CHIMICA ACTA, 2001, 443 (01) :63-72

[8]

LAVIRON E, 1974, J ELECTROANAL CHEM, V52, P355, DOI 10.1016/S0022-0728(74)80448-1

[9] GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS [J].

LAVIRON, E .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) :19-28

[10] Electrochemical detection of nitric oxide on a SWCNT/RTIL composite gel microelectrode [J].

Li, CM ;

Zang, JF ;

Zhan, DP ;

Chen, W ;

Sun, CQ ;

Teo, AL ;

Chua, YT ;

Lee, VS ;

Moochhala, SM .

ELECTROANALYSIS, 2006, 18 (07) :713-718

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