Polyphenols in the Fermentation Liquid ofDendrobium candidumRelieve Intestinal Inflammation in Zebrafish Through the Intestinal Microbiome-Mediated Immune Response

被引:43
作者
Gong, Xiaoyue [1 ]
Jiang, Shuaiming [1 ]
Tian, Haiyan [1 ]
Xiang, Dong [1 ,2 ]
Zhang, Jiachao [1 ]
机构
[1] Hainan Univ, Coll Food Sci & Engn, Haikou, Hainan, Peoples R China
[2] Hainan Univ, Key Lab Food Nutr & Funct Food Hainan Prov, Haikou, Hainan, Peoples R China
来源
FRONTIERS IN IMMUNOLOGY | 2020年 / 11卷
关键词
intestinal inflammation; intestinal microbiota; Dendrobium candidum; short-chain fatty acid; gut mucosal barrier; fermentation; CHAIN FATTY-ACIDS; GUT MICROBIOTA; DIETARY POLYPHENOLS; BARRIER FUNCTION; SYSTEM; MODEL; BUTYRATE; BACTERIA; INSIGHTS; COLITIS;
D O I
10.3389/fimmu.2020.01542
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Previous studies ofDendrobium candidum(D. candidum), which is mainly distributed in tropical areas, have mainly focused on its functional polysaccharide; the effects ofD. candidumpolyphenols, the chemical composition of which may be improved by fermentation, have received limited attention, especially inin vivomodels, which inevitably involve interactions with intestinal microorganisms. To address this challenge, metagenomic and metabolomic techniques, were applied, and immune factors and mucosal barrier-related proteins were determined to reveal the effects of fermentedD. candidumpolyphenols (FDC) on intestinal inflammation induced by oxazolone in zebrafish. The results showed that fermentation significantly changed the chemical composition ofD. candidumand that FDC significantly improved the intestinal immune index. After the 21st day of FDC intervention, the abundance ofLactobacillus, Faecalibacterium, andRummeliibacillusincreased, but the abundance of the generaShewanella, Geodermatophilus, Peptostreptococcaceae, andMycobacteriumdecreased. At the same time, FDC significantly increased intestinal short-chain fatty acids (SCFAs). In addition, network analysis based on multi-omics indicated that FDC intake leads to changes in intestinal microbiota and intestinal metabolites, resulting in enhanced host immune function. These results indicate that FDC can improve intestinal health by regulating the intestinal microbiota and its metabolites to treat intestinal inflammation and regulate the host immune system. The present research improved our understanding of the utilization ofD. candidumpolyphenols and provided new evidence for the impacts of fermentedD. candidumon host health.
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页数:15
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