Fluorescence in situ hybridization analysis of the cryptic t(12;21) (p13;q22) in childhood B-lineage acute lymphoblastic leukemia

被引：9

作者：

Yehuda-Gafni, O

Cividalli, G

Abrahmov, A

Weintrob, M

Ben Neriah, S

Cohen, R

Abeliovich, D ^{[1
]}

机构：

[1] Hadassah Hebrew Univ Hosp & Med Sch, Dept Human Genet, IL-91120 Jerusalem, Israel

[2] Hadassah Hebrew Univ Hosp & Med Sch, Dept Pediat, IL-91120 Jerusalem, Israel

[3] Shaare Zedek Med Ctr, Dept Pediat, IL-91120 Jerusalem, Israel

来源：

CANCER GENETICS AND CYTOGENETICS | 2002年 / 132卷 / 01期

关键词：

D O I：

10.1016/S0165-4608(01)00530-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We evaluated retrospectively the cryptic t(12;21)(p13;q22) in 15 children with early B-lineage acute lymphocytic leukemia who had a normal karyotype by using the locus specific probes of TEL and AML1 genes in a dual color fluorescence in situ hybridization (FISH). The FISH analysis revealed six patients with the fusion gene TEL/AML1 on chromosome 21, three of whom possessed a double fusion gene. In addition, the AML1 probe revealed hyperdiploid clones that were not detected in the conventional cytogenetic analysis. A discrepancy between the proportion of cells with the fusion gene in interphase nuclei and metaphases was noted. (C) 2002 Elsevier Science Inc. All rights reserved.

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页码：61 / 64

页数：4

相关论文

共 18 条

[1] High frequency of t(12;21) in childhood acute lymphoblastic leukemia detected by RT-PCR [J].

Amor, DJ ;

Algar, EM ;

Slater, HR ;

Smith, PJ .

PATHOLOGY, 1998, 30 (04) :381-385

[2] Cytogenetic and FISH studies of a single center consecutive series of 152 childhood acute lymphoblastic leukemias [J].

Andreasson, P ;

Höglund, M ;

Békássy, AN ;

Garwicz, S ;

Heldrup, J ;

Mitelman, F ;

Johansson, B .

EUROPEAN JOURNAL OF HAEMATOLOGY, 2000, 65 (01) :40-51

[3]

[Anonymous], 1992, Human Cytogenetics: A Practical Approach

[4] Acute lymphoblastic leukemia and chromosome 21 [J].

Berger, R .

CANCER GENETICS AND CYTOGENETICS, 1997, 94 (01) :8-12

[5] Fluorescence in situ hybridization analysis of 12;21 translocation in Japanese childhood acute lymphoblastic leukemia [J].

Eguchi-Ishimae, M ;

Eguchi, M ;

Tanaka, K ;

Hamamoto, K ;

Ohki, M ;

Ueda, K ;

Kamada, N .

JAPANESE JOURNAL OF CANCER RESEARCH, 1998, 89 (07) :783-788

[6] FUSION OF THE TEL GENE ON 12P13 TO THE AML1 GENE ON 21Q22 IN ACUTE LYMPHOBLASTIC-LEUKEMIA [J].

GOLUB, TR ;

BARKER, GF ;

BOHLANDER, SK ;

HIEBERT, SW ;

WARD, DC ;

BRAYWARD, P ;

MORGAN, E ;

RAIMONDI, SC ;

ROWLEY, JD ;

GILLILAND, DG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (11) :4917-4921

[7] Incidence of TEL/AML1 fusion gene analyzed consecutively in children with acute lymphoblastic leukemia in relapse [J].

Harbott, J ;

Viehmann, S ;

Borkhardt, A ;

Henze, G ;

Lampert, F .

BLOOD, 1997, 90 (12) :4933-4937

[8] TEL/AML-1 fusion gene: its frequency and prognostic significance in childhood acute lymphoblastic leukemia [J].

Jamil, A ;

Theil, KS ;

Kahwash, S ;

Ruymann, FB ;

Klopfenstein, KJ .

CANCER GENETICS AND CYTOGENETICS, 2000, 122 (02) :73-78

[9] An investigation of the t(12;21) rearrangement in children with B-precursor acute lymphoblastic leukaemia using cytogenetic and molecular methods [J].

Kempski, H ;

Chalker, J ;

Chessells, J ;

Sturt, N ;

Brickell, P ;

Webb, J ;

Clink, JM ;

Reeves, B .

BRITISH JOURNAL OF HAEMATOLOGY, 1999, 105 (03) :684-689

[10]

Liang DC, 1996, LEUKEMIA, V10, P991