Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

被引：22
作者：
Brion, LP
Cammer, W
Satlin, LM
Suarez, C
Zavilowitz, BJ
Schuster, VL
机构：
[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT MED, BRONX, NY 10461 USA
[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT NEUROL, BRONX, NY 10461 USA
来源：
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | 1997年 / 273卷 / 02期
关键词：
carbonic anhydrase isozymes I; II; III; and IV; carbonic anhydrase II deficiency; hydratase activity; cortex; outer medulla; liver; lung; affinity chromatography; renal tubular acidosis; regulation;
D O I：
10.1152/ajprenal.1997.273.2.F234
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) TV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA TV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.
引用
收藏
页码：F234 / F245
页数：12
相关论文
共 41 条
[1]   SUBCELLULAR-DISTRIBUTION OF CARBONIC-ANHYDRASE IN THE ENTEROCYTE OF THE RABBIT ILEUM AND THE CACO-2 CELL - EVIDENCE FOR THE PRESENCE OF 2 ISOZYMES BOUND TO BRUSH-BORDER MEMBRANES [J].
AGUENAOU, H ;
COLIN, DA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1109 (01) :59-64
[2]   A HIGH-YIELD PREPARATION FOR RAT-KIDNEY BRUSH-BORDER MEMBRANES - DIFFERENT BEHAVIOR OF LYSOSOMAL MARKERS [J].
BIBER, J ;
STIEGER, B ;
HAASE, W ;
MURER, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 647 (02) :169-176
[3]   LOCALIZATION AND ACTIVITY OF RENAL CARBONIC-ANHYDRASE (CA) IN CA-II DEFICIENT MICE [J].
BRECHUE, WF ;
KINNESAFFRAN, E ;
KINNE, RKH ;
MAREN, TH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1066 (02) :201-207
[4]   Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice [J].
Breton, S ;
Alper, SL ;
Gluck, SL ;
Sly, WS ;
Barker, JE ;
Brown, D .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 1995, 269 (06) :F761-F774
[5]  
BRION LP, 1994, J NEUROCHEM, V63, P360
[6]   METABOLIC-ACIDOSIS STIMULATES CARBONIC-ANHYDRASE ACTIVITY IN RABBIT PROXIMAL TUBULE AND MEDULLARY COLLECTING DUCT [J].
BRION, LP ;
ZAVILOWITZ, BJ ;
SUAREZ, C ;
SCHWARTZ, GJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (02) :F185-F195
[7]   MICRO-METHOD FOR THE MEASUREMENT OF CARBONIC-ANHYDRASE ACTIVITY IN CELLULAR HOMOGENATES [J].
BRION, LP ;
SCHWARTZ, JH ;
ZAVILOWITZ, BJ ;
SCHWARTZ, GJ .
ANALYTICAL BIOCHEMISTRY, 1988, 175 (01) :289-297
[8]   CHANGES IN SOLUBLE CARBONIC-ANHYDRASE ACTIVITY IN RESPONSE TO MATURATION AND NH4CL LOADING IN THE RABBIT [J].
BRION, LP ;
ZAVILOWITZ, BJ ;
ROSEN, O ;
SCHWARTZ, GJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (05) :R1204-R1213
[9]   LOCALIZATION OF MEMBRANE-ASSOCIATED CARBONIC-ANHYDRASE TYPE-IV IN KIDNEY EPITHELIAL-CELLS [J].
BROWN, D ;
ZHU, XL ;
SLY, WS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7457-7461
[10]   COMPARISON OF IMMUNOCYTOCHEMICAL STAINING OF ASTROCYTES, OLIGODENDROCYTES, AND MYELINATED FIBERS IN THE BRAINS OF CARBONIC ANHYDRASE-II-DEFICIENT MICE AND NORMAL LITTERMATES [J].
CAMMER, W ;
ZHANG, H .
JOURNAL OF NEUROIMMUNOLOGY, 1991, 34 (01) :81-86
12345