Anti-proliferative and cytotoxic effects of Strychnos nux-vomica root extract on human multiple myeloma cell line - RPMI 8226

被引:59
|
作者
Rao, Pasupuleti Sreenivasa [1 ]
Ramanadham, Madduri [2 ]
Prasad, Majeti Narasimha Vara [1 ]
机构
[1] Univ Hyderabad, Sch Life Sci, Dept Plant Sci, Lab Environm Biotechnol, Hyderabad 500046, Andhra Pradesh, India
[2] Univ Hyderabad, Sch Life Sci, Dept Biochem, Cellular Immunol Lab, Hyderabad 500046, Andhra Pradesh, India
关键词
Strychnos nux-vomica; Strychnine; Brucine; Apoptosis; Multiple myeloma; INDUCED APOPTOSIS; CYTOCHROME-C; ALKALOIDS; TOXICITY; LEUKEMIA; BRUCINE; SEEDS;
D O I
10.1016/j.fct.2008.10.027
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Multiple myeloma (MM) is an incurable hematological malignancy with high incidence in the elderly. The currently used chemotherapeutic drugs show severe side effects, dose-limiting toxicity and development of resistance. In search of novel plant derived anti-cancer agents, Strychnos nux-vomica L. (SN) root extract was screened using the human MM-cell line, RPMI 8226. SN-extract exhibited anti-proliferative activity in a dose and time dependent manner. The morphological assessment of SN-extract treated cells showed significant features associated with apoptosis. Cell cycle analysis using flow cytometry of cells stained with propidium iodide revealed accumulation of cells at sub-G(0)/G(1) phase. In addition, disruption of mitochondrial membrane potential and subsequent leakage of mitorchondrial cytochrome c was observed in SN-extract treated myeloma cells. The anti-proliferative and cytotoxic activity could be due to the alkaloids strychnine and brucine, which have been identified by LC-mass spectral analysis of the SN-extract in comparison to the reference standards analyzed under identical conditions. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:283 / 288
页数:6
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