Technologies and Standardization in Research on Extracellular Vesicles

被引：359
作者：
Gandham, Srujan [1 ]
Su, Xianyi [2 ]
Wood, Jacqueline [2 ]
Nocera, Angela L. [1 ]
Alli, Sarath Chandra [2 ,3 ]
Milane, Lara [1 ]
Zimmerman, Alan [2 ]
Amiji, Mansoor [1 ]
Ivanov, Alexander R. [2 ]
机构：
[1] Northeastern Univ, Sch Pharm, Dept Pharmaceut Sci, Boston, MA 02115 USA
[2] Northeastern Univ, Barnett Inst Chem & Biol Anal, Dept Chem & Chem Biol, Boston, MA 02115 USA
[3] Northeastern Univ, Dept Bioengn, Boston, MA 02115 USA
来源：
TRENDS IN BIOTECHNOLOGY | 2020年 / 38卷 / 10期
基金：
美国国家卫生研究院;
关键词：
DENSITY-GRADIENT SEPARATION; CELL-DERIVED EXOSOMES; MASS-SPECTROMETRY; OVARIAN-CANCER; PROTEOMICS ANALYSIS; PURIFIED EXOSOMES; LIGHT-SCATTERING; TUMOR-GROWTH; PROTEIN; RNA;
D O I：
10.1016/j.tibtech.2020.05.012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids. EV-mediated transfer of biomolecules is a critical component of a variety of physiological and pathological processes. Potential applications of EVs in novel diagnostic and therapeutic strategies have brought increasing attention. However, EV research remains highly challenging due to the inherently complex biogenesis of EVs and their vast heterogeneity in size, composition, and origin. There is a need for the establishment of standardized methods that address EV heterogeneity and sources of pre-analytical and analytical variability in EV studies. Here, we review technologies developed for EV isolation and characterization and discuss paths toward standardization in EV research.
引用
收藏
页码：1066 / 1098
页数：33
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