Cellulose Nanofiber-Reinforced Chitosan Hydrogel Composites for Intervertebral Disc Tissue Repair

被引：77

作者：

Doench, Ingo ^{[1
,2
]}

Tuan Ahn Tran ^{[1
,2
]}

David, Laurent ^{[3
]}

Montembault, Alexandra ^{[3
]}

Viguier, Eric ^{[4
]}

Gorzelanny, Christian ^{[5
]}

Sudre, Guillaume ^{[3
]}

Cachon, Thibaut ^{[4
]}

Louback-Mohamed, Malika ^{[1
,2
,3
]}

Horbelt, Niels ^{[6
]}

Peniche-Covas, Carlos ^{[7
]}

Osorio-Madrazo, Anayancy ^{[1
,2
]}

机构：

[1] Univ Freiburg, Inst Microsyst Engn IMTEK, Lab Sensors, D-79110 Freiburg, Germany

[2] Univ Freiburg, Freiburg Mat Res Ctr FMF, D-79104 Freiburg, Germany

[3] Univ Lyon, Univ Claude Bernard Lyon 1, CNRS UMR 5223, IMP, F-69622 Villeurbanne, France

[4] Univ Lyon, VetAgro Sup, ICE, F-69280 Marcy Letoile, France

[5] Univ Med Ctr Hamburg Eppendorf, Dept Dermatol & Venerol, D-20246 Hamburg, Germany

[6] Max Planck Inst Colloids & Interfaces, Biomat Dept, Sci Pk Golm, D-14476 Potsdam, Germany

[7] Univ Havana, Ctr Biomat, Havana 10600, Cuba

来源：

BIOMIMETICS | 2019年 / 4卷 / 01期

关键词：

hydrogel biomaterial; chitosan; cellulose nanofibers; intervertebral disc; tissue engineering; NANOCOMPOSITES; NANOWHISKERS; REPLACEMENT; EFFICIENCY; SCAFFOLD; CLOSURE; CELLS;

D O I：

10.3390/biomimetics4010019

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The development of non-cellularized composites of chitosan (CHI) hydrogels, filled with cellulose nanofibers (CNFs) of the type nanofibrillated cellulose, was proposed for the repair and regeneration of the intervertebral disc (IVD) annulus fibrosus (AF) tissue. With the achievement of CNF-filled CHI hydrogels, biomaterial-based implants were designed to restore damaged/degenerated discs. The structural, mechanical and biological properties of the developed hydrogel composites were investigated. The neutralization of weakly acidic aqueous CNF/CHI viscous suspensions in NaOH yielded composites of physical hydrogels in which the cellulose nanofibers reinforced the CHI matrix, as investigated by means of microtensile testing under controlled humidity. We assessed the suitability of the achieved biomaterials for intervertebral disc tissue engineering in ex vivo experiments using spine pig models. Cellulose nanofiber-filled chitosan hydrogels can be used as implants in AF tissue defects to restore IVD biomechanics and constitute contention patches against disc nucleus protrusion while serving as support for IVD regeneration.

引用

页数：17

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