DNA damage in children and adolescents with cardiovascular disease risk factors

被引:9
作者
Kliemann, Mariele [1 ]
Pra, Daniel [2 ,3 ]
Mueller, Luiza L. [4 ]
Hermes, Liziane [4 ]
Horta, Jorge A. [2 ]
Reckziegel, Miriam B. [2 ]
Burgos, Miria S. [2 ]
Maluf, Sharbel W. [5 ]
Franke, Silvia I. R. [2 ,4 ]
da Silva, Juliana [1 ]
机构
[1] Univ Luterana Brasil, Programa Posgrad Biol Celular & Mol Aplicada Saud, BR-92425900 Canoas, RS, Brazil
[2] Univ Santa Cruz do Sul, Programa Posgrad Promocao Saude, BR-96815900 Santa Cruz Do Sul, RS, Brazil
[3] Univ Catolica Pelotas, Programa Posgrad Saude & Comportamento, BR-96010280 Pelotas, RS, Brazil
[4] Univ Santa Cruz Do Sul, DEDFIS, BR-96815900 Santa Cruz Do Sul, RS, Brazil
[5] Hosp Clin Porto Alegre, Serv Genet Med, BR-90035903 Porto Alegre, RS, Brazil
来源
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2012年 / 84卷 / 03期
关键词
cardiovascular diseases; Micronucleus test; Comet assay; diet; anthropometry; child; adolescent; DETAILED DESCRIPTION; OXIDATIVE STRESS; VITAMIN-C; MICRONUCLEUS; FOLATE; ASSAY; GENOTOXICITY; MECHANISMS; PREVENTION; IMPACT;
D O I
10.1590/S0001-37652012005000039
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P<0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.
引用
收藏
页码:833 / 840
页数:8
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