Cellular Mechanics of Modulated Osteoblasts Functions in Graphene Oxide Reinforced Elastomers

被引：47

作者：

Girase, Bhupendra ^{[1
]}

Shah, Jinesh S. ^{[2
]}

Misra, R. Devesh K. ^{[1
]}

机构：

[1] Univ Louisiana Lafayette, Biomat & Biomed Engn Res Lab, Ctr Struct & Funct Mat, Lafayette, LA 70504 USA

[2] Global Nanotech, Bombay 400062, Maharashtra, India

来源：

ADVANCED ENGINEERING MATERIALS | 2012年 / 14卷 / 04期

关键词：

WALLED CARBON NANOTUBES; CALCIUM-PHOSPHATE; BONE; FIBRONECTIN; SCAFFOLDS; SILICONE; ADHESION; CELLS; BIOCOMPATIBILITY; NANOCOMPOSITES;

D O I：

10.1002/adem.201180028

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We describe here favorable modulation of osteoblasts functions and cellsubstrate interactions in hybrid silicone elastomers consisting of biocompatible graphene oxide. Pressure induced curing was used to synthesize the hybrid silicone elastomer with high strengthhigh elongation combination. It was intriguing that the cellsubstrate interactions in the hybrid silicone elastomer were observed to be significantly different from those observed in stand alone silicone. The origin of differences in cellsubstrate interactions in terms of cell attachment, viability, and proliferation and assessment of proteins actin, vinculin, and fibronectin are addressed and attributed to physico-chemical properties (topography and hydrophilicity) and to the presence of graphene oxide. The end outcome of the study is a new family of nanostructured polymer composite with desired (enhanced cell functions) and bulk properties (long term stabilityhigh strength-at-break). The integration of cellular and molecular biology with material science and engineering described here provides an insight into the ability to modulate cellular and molecular reactions in promoting osteoinductive signaling of surface adherent cells, in the present case, osteoblasts for joint reconstruction.

引用

页码：B101 / B111

页数：11

共 60 条

[1] FACTORS THAT PROMOTE PROGRESSIVE DEVELOPMENT OF THE OSTEOBLAST PHENOTYPE IN CULTURED FETAL-RAT CALVARIA CELLS [J].

ARONOW, MA ;

GERSTENFELD, LC ;

OWEN, TA ;

TASSINARI, MS ;

STEIN, GS ;

LIAN, JB .

JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 143 (02) :213-221

[2] Carbon nanotubes as structural nanofibers for hyaluronic acid hydrogel scaffolds [J].

Bhattacharyya, Sanjib ;

Guillott, Samuel ;

Dabboue, Hinda ;

Tranchant, Jean-Francois ;

Salvetat, Jean-Paul .

BIOMACROMOLECULES, 2008, 9 (02) :505-509

[3] Electrically polarized HAp-coated Ti: In vitro bone cell-material interactions [J].

Bodhak, Subhadip ;

Bose, Susmita ;

Bandyopadhyay, Amit .

ACTA BIOMATERIALIA, 2010, 6 (02) :641-651

[4]

Catanese J, 1999, J BIOMED MATER RES, V48, P187, DOI 10.1002/(SICI)1097-4636(1999)48:2<187::AID-JBM13>3.3.CO

[5]

2-D

[6] Mechanically strong, electrically conductive, and biocompatible graphene paper [J].

Chen, Haiqun ;

Mueller, Marc B. ;

Gilmore, Kerry J. ;

Wallace, Gordon G. ;

Li, Dan .

ADVANCED MATERIALS, 2008, 20 (18) :3557-+

[7] STUDIES ON INTER-CELLULAR LETS GLYCOPROTEIN MATRICES [J].

CHEN, LB ;

MURRAY, A ;

SEGAL, RA ;

BUSHNELL, A ;

WALSH, ML .

CELL, 1978, 14 (02) :377-391

[8] In vitro behavior of osteoblast-like cells on fluoridated hydroxyapatite coatings [J].

Cheng, K ;

Weng, WJ ;

Wang, HM ;

Zhang, S .

BIOMATERIALS, 2005, 26 (32) :6288-6295

[9] Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells [J].

Cherukuri, P ;

Bachilo, SM ;

Litovsky, SH ;

Weisman, RB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (48) :15638-15639

[10] Mammalian pharmacokinetics of carbon nanotubes using intrinsic near-infrared fluorescence [J].

Cherukuri, Paul ;

Gannon, Christopher J. ;

Leeuw, Tonya K. ;

Schmidt, Howard K. ;

Smalley, Richard E. ;

Curley, Steven A. ;

Weisman, R. Bruce .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (50) :18882-18886

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