Electrochemical immunoassay of benzo[a]pyrene based on dual amplification strategy of electron-accelerated Fe3O4/polyaniline platform and multi-enzyme-functionalized carbon sphere label

被引：39
作者：
Lin, Mouhong [1 ]
Liu, Yingju [1 ]
Sun, Zihong [1 ]
Zhang, Shenglai [1 ]
Yang, Zhuohong [1 ]
Ni, Chunlin [1 ]
机构：
[1] S China Agr Univ, Coll Sci, Inst Biomat, Guangzhou 510642, Guangdong, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2012年 / 722卷
关键词：
Immunosensor; Benzo[a]pyrene; Fe3O4/polyaniline; Carbon nanospheres; FOOD SAMPLES; NANOPARTICLES; IMMUNOSENSOR; GRAPHENE;
D O I：
10.1016/j.aca.2012.01.059
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An electrochemical immunosensor, basing on a dual amplification strategy by employing a biocompatible Fe3O4/polyaniline/Nafion (Fe3O4/PANI/Nafion) layer as sensor platform and multi-enzyme-antibody functionalized highly-carbonized spheres (multi-HRP-HCS-Ab(2)) as label, was constructed for sensitive detection of benzo[a]pyrene (BaP). The stable film, Fe3O4/PANI/Nafion, can not only immobilize biomolecules, but also catalyze the reduction of hydrogen peroxide, indicating an accelerated electron transfer pathway of the platform. The experimental conditions, including the concentration of Nation, concentration of Fe3O4/polyaniline (Fe3O4/PANI), pH of the detection solution and concentrations of biomolecules, were studied in detail. Basing on a competitive immunoassay, the current change was proportional to the logarithm of BaP concentration in the range of 8 pM and 2 nM with the detection limit of 4pM. The proposed immunosensor exhibited acceptable reproducibility and stability. This new type of dual amplification strategy may provide potential applications for the detection of environmental pollutants. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：100 / 106
页数：7
相关论文
共 35 条
[1]   Surface IR immunosensors for label-free detection of benzo[a]pyrene [J].
Boujday, Souhir ;
Nasri, Soumaya ;
Salmain, Michele ;
Pradier, Claire-Marie .
BIOSENSORS & BIOELECTRONICS, 2010, 26 (04) :1750-1754
[2]   Nanogold-actuated biomimetic peroxidase for sensitized electrochemical immunoassay of carcinoembryonic antigen in human serum [J].
Chen, Huafeng ;
Tang, Juan ;
Su, Biting ;
Chen, Guonan ;
Huang, Jianxin ;
Tang, Dianping .
ANALYTICA CHIMICA ACTA, 2010, 678 (02) :169-175
[3]   Functionalized Graphene Oxide as a Nanocarrier in a Multienzyme Labeling Amplification Strategy for Ultrasensitive Electrochemical Immunoassay of Phosphorylated p53 (S392) [J].
Du, Dan ;
Wang, Limin ;
Shao, Yuyan ;
Wang, Jun ;
Engelhard, Mark H. ;
Lin, Yuehe .
ANALYTICAL CHEMISTRY, 2011, 83 (03) :746-752
[4]   Amphiphile Disruption of Pathogen Attachment at the Hematite (α-Fe2O3)-Water Interface [J].
Gao, Xiaodong ;
Chorover, Jon .
LANGMUIR, 2011, 27 (10) :5936-5943
[5]   Double-Miniemulsion Preparation of Fe3O4/Poly(methyl methacrylate) Magnetic Latex [J].
Hong, R. Y. ;
Feng, B. ;
Cai, X. ;
Liu, G. ;
Li, H. Z. ;
Ding, J. ;
Zheng, Y. ;
Wei, D. G. .
JOURNAL OF APPLIED POLYMER SCIENCE, 2009, 112 (01) :89-98
[6]   Piezoelectric immunosensor with gold nanoparticles enhanced competitive immunoreaction technique for quantification of aflatoxin B1 [J].
jin, Xiaoyong ;
Jin, Xuefang ;
Chen, Liguo ;
Jiang, Jianhui ;
Shen, Guoli ;
Yu, Ruqin .
BIOSENSORS & BIOELECTRONICS, 2009, 24 (08) :2580-2585
[7]   Attomolar Detection of a Cancer Biomarker Protein in Serum by Surface Plasmon Resonance Using Superparamagnetic Particle Labels [J].
Krishnan, Sadagopan ;
Mani, Vigneshwaran ;
Wasalathanthri, Dhanuka ;
Kumar, Challa V. ;
Rusling, James F. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (05) :1175-1178
[8]   Nanofibers of conducting polyaniline for aromatic organic compound sensor [J].
Li, Wei ;
Hoa, Nguyen Duc ;
Cho, Yousuk ;
Kim, Dojin ;
Kim, Jeong-Soo .
SENSORS AND ACTUATORS B-CHEMICAL, 2009, 143 (01) :132-138
[9]   Preparation and properties of poly(acrylic acid) oligomer stabilized superparamagnetic ferrofluid [J].
Lin, CL ;
Lee, CF ;
Chiu, WY .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2005, 291 (02) :411-420
[10]   A wireless and sensitive sensing detection of polycyclic aromatic hydrocarbons using humic acid-coated magnetic Fe3O4 nanoparticles as signal-amplifying tags [J].
Lin, Hailan ;
Chen, Zhang ;
Lu, Qingzhu ;
Cai, Qingyun ;
Grimes, Craig A. .
SENSORS AND ACTUATORS B-CHEMICAL, 2010, 146 (01) :154-159
1234