Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid loach, Misgurnus anguillicaudatus

被引：9
作者：
Zhao, Y. [1 ]
Psenicka, M. [2 ]
Fujimoto, T. [1 ]
Saito, T. [3 ]
Yasui, G. S. [1 ]
Yamaha, E. [3 ]
Arai, K. [1 ]
机构：
[1] Hokkaido Univ, Fac & Grad Sch Fisheries Sci, Hakodate, Hokkaido 0418611, Japan
[2] Univ S Bohemia, S Bohemian Res Ctr Aquaculture & Biodivers Hydroc, Fac Fisheries & Protect Waters, Vodnany, Zatisi, Czech Republic
[3] Hokkaido Univ, Nanae Fresh Water Lab, Field Sci Ctr No Biosphere, Nanae, Hokkaido, Japan
来源：
JOURNAL OF APPLIED ICHTHYOLOGY | 2012年 / 28卷 / 06期
关键词：
INTRACYTOPLASMIC SPERM INJECTION; INTERSPECIFIC HYBRIDIZATION; PACIFIC OYSTERS; RAINBOW-TROUT; CRUCIAN CARP; FISH; ULTRASTRUCTURE; EGGS; FERTILIZATION; AQUACULTURE;
D O I：
10.1111/jai.12069
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Diploid spermatozoa were obtained from sex-reversed clonal diploid males and neo-tetraploid which derived from normal diploid x natural tetraploid cross followed by the 2nd polar body release inhibition. The spermatozoa from sex-reversed clonal diploid males exhibited a significant reduction in motility when compared with those from normal diploid and neo-tetraploid males. Electron microscopy revealed that diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid males exhibited normal shape similar to haploid spermatozoa from normal diploid males, except for larger sperm-head size and longer flagellum. Considering flow-cytometric results, volume of mitochondrial mass per spermatozoon was also increased in proportion to the elevation of ploidy status from haploid to diploid sperm. Diploid spermatozoa from neo-tetraploid increased the number of mitochondria and the ATP content, but those from sex-reversed clonal diploid males did not show such results.
引用
收藏
页码：1006 / 1012
页数：7
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