Culture of primary rat hippocampal neurons: design, analysis, and optimization of a microfluidic device for cell seeding, coherent growth, and solute delivery

被引：6

作者：

Barbati, Alexander C. ^{[1
]}

Fang, Cheng ^{[2
]}

Banker, Gary A. ^{[2
]}

Kirby, Brian J. ^{[1
]}

机构：

[1] Cornell Univ, Sibley Sch Mech & Aerosp Engn, Ithaca, NY 14853 USA

[2] Oregon Hlth & Sci Univ, Jungers Ctr Neurosci Res, Portland, OR 97201 USA

来源：

BIOMEDICAL MICRODEVICES | 2013年 / 15卷 / 01期

基金：

美国国家科学基金会;

关键词：

Drug delivery; Microfluidic; Cell culture; Axonal transport; Axonal damage; Neuron; Neurodegenerative disease; PDMS; Huntington's disease; LOCAL-CONTROL; COMPARTMENTALIZED CULTURES; SYMPATHETIC NEURONS; TRANSPORT; ARRAYS; GRADIENTS; MOLECULES; DISEASES; SYSTEMS; SCALE;

D O I：

10.1007/s10544-012-9691-2

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We present the design, analysis, construction, and culture results of a microfluidic device for the segregation and chemical stimulation of primary rat hippocampal neurons. Our device is designed to achieve spatio-temporal solute delivery to discrete sections of neurons with mitigated mechanical stress. We implement a geometric guidance technique to direct axonal processes of the neurons into specific areas of the device to achieve solute segregation along routed cells. Using physicochemical modeling, we predict flows, concentration profiles, and mechanical stresses within pertiment sections of the device. We demonstrate cell viability and growth within the closed device over a period of 11 days. Additionally, our modeling methodology may be generalized and applied to other device geometries.

引用

页码：97 / 108

页数：12

共 31 条

[1]

Banker G., 1998, CULTURING NERVE CELL

[2] DEVELOPMENT OF SYMPATHETIC NEURONS IN COMPARTMENTALIZED CULTURES .2. LOCAL-CONTROL OF NEURITE SURVIVAL BY NERVE GROWTH-FACTOR [J].

CAMPENOT, RB .

DEVELOPMENTAL BIOLOGY, 1982, 93 (01) :13-21

[3] LOCAL CONTROL OF NEURITE DEVELOPMENT BY NERVE GROWTH-FACTOR [J].

CAMPENOT, RB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (10) :4516-4519

[4] DEVELOPMENT OF SYMPATHETIC NEURONS IN COMPARTMENTALIZED CULTURES .1. LOCAL-CONTROL OF NEURITE GROWTH BY NERVE GROWTH-FACTOR [J].

CAMPENOT, RB .

DEVELOPMENTAL BIOLOGY, 1982, 93 (01) :1-12

[5] Role of axonal transport in neurodegenerative diseases [J].

De Vos, Kurt J. ;

Grierson, Andrew J. ;

Ackerley, Steven ;

Miller, Christopher C. J. .

ANNUAL REVIEW OF NEUROSCIENCE, 2008, 31 :151-173

[6] Applications of microfluidics for neuronal studies [J].

Gross, Pamela G. ;

Kartalov, Emil P. ;

Scherer, Axel ;

Weiner, Leslie P. .

JOURNAL OF THE NEUROLOGICAL SCIENCES, 2007, 252 (02) :135-143

[7] Aligned microcontact printing of micrometer-scale poly-L-lysine structures for controlled growth of cultured neurons on planar microelectrode arrays [J].

James, CD ;

Davis, R ;

Meyer, M ;

Turner, A ;

Turner, S ;

Withers, G ;

Kam, L ;

Banker, G ;

Craighead, H ;

Isaacson, M ;

Turner, J ;

Shain, W .

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2000, 47 (01) :17-21

[8]

Kaech S., 2012, COLD SPRING HARBOR P, V2012, DOI [10.1101/pdb.prot068247, DOI 10.1101/PDB.PROT068247]

[9] Culturing hippocampal neurons [J].

Kaech, Stefanie ;

Banker, Gary .

NATURE PROTOCOLS, 2006, 1 (05) :2406-2415

[10] Biomolecular gradients in cell culture systems [J].

Keenan, Thomas M. ;

Folch, Albert .

LAB ON A CHIP, 2008, 8 (01) :34-57

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