Simultaneous determination of 19 intracellular nucleotides and nucleotide sugars in Chinese Hamster ovary cells by capillary electrophoresis

被引：43

作者：

Feng, Hua-Tao ^{[1
]}

Wong, Niki ^{[1
]}

Wee, Sheena ^{[1
]}

Lee, May May ^{[1
]}

机构：

[1] ASTAR, Bioproc Technol Inst, Singapore 138668, Singapore

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 870卷 / 01期

关键词：

capillary electrophoresis; intracellular; CHO cells; nucleotides; nucleotide sugars;

D O I：

10.1016/j.jchromb.2008.05.038

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Twelve nucleotides and seven nucleotide sugars in Chinese Hamster ovary (CHO) cells were determined by capillary electrophoresis (CE). The CE operating conditions of buffer pH value, ion strength, capillary temperature, polymer additive and cell extraction method were investigated. Optimum separation was achieved with 40 mM sodium tetraborate buffer (pH 9.5) containing 1% (w/v) polyethylene glycol (PEG) at a capillary temperature of 22 degrees C. Acetonitrile and chloroform were used for intracellular extraction. This method can be used to monitor intracellular carbohydrate metabolism. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：131 / 134

页数：4

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