Detection of Helicobacter pylori by PCR on gastric biopsy specimens taken for CP test:: Comparison with histopathological analysis

被引:7
作者
Di Bonaventura, G
Neri, M
Angelucci, D
Rosini, S
Piccolomini, M
Piccolomini, R
机构
[1] Univ G DAnnunzio, Lab Clin Microbiol, Dept Biomed Sci, Sch Med, I-66100 Chieti, Italy
[2] Univ G DAnnunzio, Ctr Excellence Aging, Sch Med, I-66100 Chieti, Italy
[3] Univ G DAnnunzio, Gastroenterol Serv, Sch Med, Dept Med & Aging Sci, Chieti, Italy
[4] Univ G DAnnunzio, Histopathol Serv, Dept Oncol & Neurosci, Chieti, Italy
来源
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY | 2004年 / 17卷 / 01期
关键词
Helicobacter pylori; rapid urease test; PCR;
D O I
10.1177/039463200401700111
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aims of the present study were: (i) to assess whether H. pylori could be succesfully detected by PCR from the same biopsy sample used for CPtest; and ii) to evaluate CPtest comparatively to both PCR and histology for detection of H. pylori infection in dyspeptic patients. Three antral gastric biopsies were collected from each of 80 consecutive dyspeptic patients undergoing oesophago-gastroduodenoscopy. Two biopsies were for histology (gold standard), one for CPtest, scored at 20min, 1h and 24h for the presence of urease activity. Gastric biopsy was then removed from CPtest and used for ureC-targeted PCR. Fifty-five (68.7%) patients were positive for H. pylori infection by histology. CPtest yielded an overall diagnostic accuracy of 93.8% (95% CI: 91-96.4%), regardless of observation period. No erroneous categorization of H. pylori status occurred using PCR, yielding sensitivity, specificity, positive and negative predictive values, and overall diagnostic accuracy of 100%. Our results suggest that H. pylori can be detected by PCR in gastric biopsies previously taken for CPtest, so reducing the workload of the endoscopist by saving additional biopsies for culture analysis and susceptibility tests.
引用
收藏
页码:77 / 82
页数:6
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