Three pentacyclic triterpenes protect H9c2 cardiomyoblast cells against high-glucose-induced injury

H9c2 cardiomyoblast cell line was used to examine the protection of three triterpenes, asiatic acid, boswellic acid, and oleanolic acid, at 5 or 10 mu M against high-glucose-induced injury. High glucose stimulated reactive oxygen species (ROS), oxidized glutathione (GSSG), interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 production, as well as decreased glutathione peroxidase (GPX), glutathione reductase (GR) and catalase activities, and protein expression. However, pre-treatments of three triterpenes reserved glutathione, maintained activity and expression of GPX, GR, and catalase, as well as lowered ROS, GSSG, and inflammatory cytokines generation. High glucose reduced Na+ -K+ -ATPase activity, raised nuclear factor kappa (NF-kappa) B and caspase-3 activities, up-regulated protein expression of NF-kappa. B, mitogen-activated protein kinase, Bax, and cleaved caspase-3, as well as down-regulated Bcl-2 expression. Pre-treatments of three triterpenes retained Na+ -K+ -ATPase activity, declined NF-kappa B and caspase-3 activities, reserved Bcl-2 expression, as well as suppressed protein expression of NF-kappa B, p-p38, Bax, and cleaved caspase-3. These findings suggest that these triterpenes are potent cardiac-protective agents.