Biological Regulation via Ankyrin Repeat Folding

被引：7

作者：

Barrick, Doug ^{[1
]}

机构：

[1] Johns Hopkins Univ, TC Jenkins Dept Biophys, Baltimore, MD 21218 USA

来源：

ACS CHEMICAL BIOLOGY | 2009年 / 4卷 / 01期

关键词：

CDK INHIBITOR P19(INK4D); KAPPA-B-ALPHA; CRYSTAL-STRUCTURE; CELL-CYCLE; PROTEIN; NOTCH; STABILITY; COMPLEX; DOMAIN; COOPERATIVITY;

D O I：

10.1021/cb900003f

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By mimicking the phosphorylation of p19(INK4d), a tumor suppressor containing five ankyrin repeats, the native state could be destabilized to such an extent that only a partially folded state is populated at physiological temperature. This partly folded state, which mimics an on-pathway folding intermediate lacking structure in ankyrin repeats 1 and 2, is more rapidly ubiquitinated than the parent construct. Thus, phosphorylation of p(19INK4d) is likely to regulate cell-cycle progression through both biochemical (proteasomal) and biophysical (folding and binding to cyclin-dependent kinases) mechanisms.

引用

页码：19 / 22

页数：4

共 22 条

[1] Crystal structure of the complex of the cyclin D dependent kinase Cdk6 bound to the cell-cycle inhibitor p19INK4d [J].

Brotherton, DH ;

Dhanaraj, V ;

Wick, S ;

Brizuela, L ;

Domaille, PJ ;

Volyanik, E ;

Xu, X ;

Parisini, E ;

Smith, BO ;

Archer, SJ ;

Serrano, M ;

Brenner, SL ;

Blundell, TL ;

Laue, ED .

NATURE, 1998, 395 (6699) :244-250

[2] Asparaginyl hydroxylation of the notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor [J].

Coleman, Mathew L. ;

McDonough, Michael A. ;

Hewitson, Kirsty S. ;

Coles, Charlotte ;

Mecinovic, Jasmin ;

Edelmann, Mariola ;

Cook, Kristina M. ;

Cockman, Matthew E. ;

Lancaster, David E. ;

Kessler, Benedikt M. ;

Oldham, Neil J. ;

Ratcliffe, Peter J. ;

Schofield, Christopher J. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (33) :24027-24038

[3]

DeLano WL., 2003, MacPyMOL: PyMOL Enhanced for Mac OS X

[4] Intrinsically unstructured proteins and their functions [J].

Dyson, HJ ;

Wright, PE .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (03) :197-208

[5] Asparagine β-hydroxylation stabilizes the ankyrin repeat domain fold [J].

Kelly, Leanne ;

McDonough, Michael A. ;

Coleman, Mathew L. ;

Ratcliffe, Peter J. ;

Schofield, Christopher J. .

MOLECULAR BIOSYSTEMS, 2009, 5 (01) :52-58

[6] Repeat-protein folding: New insights into origins of cooperativity, stability, and topology [J].

Kloss, Ellen ;

Courtemanche, Naomi ;

Barrick, Doug .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2008, 469 (01) :83-99

[7] COMPARISON OF EXPERIMENTAL BINDING DATA AND THEORETICAL MODELS IN PROTEINS CONTAINING SUBUNITS [J].

KOSHLAND, DE ;

NEMETHY, G ;

FILMER, D .

BIOCHEMISTRY, 1966, 5 (01) :365-+

[8] Folding mechanism of an ankyrin repeat protein:: Scaffold and active site formation of human CDK inhibitor p19INK4d [J].

Loew, Christian ;

Weininger, Ulrich ;

Zeeb, Markus ;

Zhang, Wei ;

Laue, Ernest D. ;

Schmid, Franz X. ;

Balbach, Jochen .

JOURNAL OF MOLECULAR BIOLOGY, 2007, 373 (01) :219-231

[9] Conformational Switch upon Phosphorylation: Human CDK Inhibitor p19INK4d between the Native and Partially Folded State [J].

Loew, Christian ;

Homeyer, Nadine ;

Weininger, Ulrich ;

Sticht, Heinrich ;

Balbach, Jochen .

ACS CHEMICAL BIOLOGY, 2009, 4 (01) :53-63

[10] Dissection of protein-protein interaction and CDK4 inhibition in the oncogenic versus tumor suppressing functions of gankyrin and P16 [J].

Mahajan, Anjali ;

Guo, Y. ;

Yuan, Chunhua ;

Weghorst, Christopher M. ;

Tsai, Ming-Daw ;

Li, Junan .

JOURNAL OF MOLECULAR BIOLOGY, 2007, 373 (04) :990-1005

← 1 2 3 →