IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells

被引：22

作者：

Blasi, Maria ^{[1
,2
]}

Negri, Donatella ^{[1
,2
,3
]}

LaBranche, Celia ^{[4
]}

Alam, S. Munir ^{[1
,2
,5
]}

Baker, Erich J. ^{[1
,2
]}

Brunner, Elizabeth C. ^{[1
,2
]}

Gladden, Morgan A. ^{[2
]}

Michelini, Zuleika ^{[3
]}

Vandergrift, Nathan A. ^{[1
,2
]}

Wiehe, Kevin J. ^{[1
,2
]}

Parks, Robert ^{[1
,2
]}

Shen, Xiaoying ^{[1
,2
]}

Bonsignori, Mattia ^{[2
]}

Tomaras, Georgia D. ^{[2
,4
]}

Ferrari, Guido ^{[4
]}

Montefiori, David C. ^{[4
]}

Santra, Sampa ^{[6
]}

Haynes, Barton F. ^{[1
,2
]}

Moody, Michael A. ^{[2
,7
]}

Cara, Andrea ^{[1
,2
,3
]}

Klotman, Mary E. ^{[1
,2
]}

机构：

[1] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA

[2] Duke Univ, Med Ctr, Duke Human Vaccine Inst, Durham, NC 27710 USA

[3] Ist Super Sanita, I-00161 Rome, Italy

[4] Duke Univ, Med Ctr, Dept Surg, Durham, NC 27710 USA

[5] Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA

[6] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA

[7] Duke Univ, Med Ctr, Dept Pediat, Durham, NC 27710 USA

来源：

COMMUNICATIONS BIOLOGY | 2018年 / 1卷

基金：

美国国家卫生研究院;

关键词：

DEFECTIVE LENTIVIRAL VECTOR; HIV-1; VACCINE; NEUTRALIZING ANTIBODIES; DENDRITIC CELLS; T-CELL; RESPONSES; IMMUNIZATION; IMMUNOGENICITY; EXPRESSION; EFFICIENT;

D O I：

10.1038/s42003-018-0131-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

HIV continues to be a major global health issue. In spite of successful prevention interventions and treatment methods, the development of an HIV vaccine remains a major priority for the field and would be the optimal strategy to prevent new infections. We showed previously that a single immunization with a SIV-based integrase-defective lentiviral vector (IDLV) expressing the 1086.C HIV-1-envelope induced durable, high-magnitude immune responses in non-human primates (NHPs). In this study, we have further characterized the humoral responses by assessing antibody affinity maturation and antigen-specific memory B-cell persistence in two vaccinated macaques. These animals were also boosted with IDLV expressing the heterologous 1176.C HIV-1-Env to determine if neutralization breadth could be increased, followed by evaluation of the injection sites to assess IDLV persistence. IDLV-Env immunization was associated with persistence of the vector DNA for up to 6 months post immunization and affinity maturation of antigen-specific memory B cells.

引用

页数：13

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