IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells

被引：21

作者：

Blasi, Maria ^{[1
,2
]}

Negri, Donatella ^{[1
,2
,3
]}

LaBranche, Celia ^{[4
]}

Alam, S. Munir ^{[1
,2
,5
]}

Baker, Erich J. ^{[1
,2
]}

Brunner, Elizabeth C. ^{[1
,2
]}

Gladden, Morgan A. ^{[2
]}

Michelini, Zuleika ^{[3
]}

Vandergrift, Nathan A. ^{[1
,2
]}

Wiehe, Kevin J. ^{[1
,2
]}

Parks, Robert ^{[1
,2
]}

Shen, Xiaoying ^{[1
,2
]}

Bonsignori, Mattia ^{[2
]}

Tomaras, Georgia D. ^{[2
,4
]}

Ferrari, Guido ^{[4
]}

Montefiori, David C. ^{[4
]}

Santra, Sampa ^{[6
]}

Haynes, Barton F. ^{[1
,2
]}

Moody, Michael A. ^{[2
,7
]}

Cara, Andrea ^{[1
,2
,3
]}

Klotman, Mary E. ^{[1
,2
]}

机构：

[1] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA

[2] Duke Univ, Med Ctr, Duke Human Vaccine Inst, Durham, NC 27710 USA

[3] Ist Super Sanita, I-00161 Rome, Italy

[4] Duke Univ, Med Ctr, Dept Surg, Durham, NC 27710 USA

[5] Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA

[6] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA

[7] Duke Univ, Med Ctr, Dept Pediat, Durham, NC 27710 USA

来源：

COMMUNICATIONS BIOLOGY | 2018年 / 1卷

基金：

美国国家卫生研究院;

关键词：

DEFECTIVE LENTIVIRAL VECTOR; HIV-1; VACCINE; NEUTRALIZING ANTIBODIES; DENDRITIC CELLS; T-CELL; RESPONSES; IMMUNIZATION; IMMUNOGENICITY; EXPRESSION; EFFICIENT;

D O I：

10.1038/s42003-018-0131-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

HIV continues to be a major global health issue. In spite of successful prevention interventions and treatment methods, the development of an HIV vaccine remains a major priority for the field and would be the optimal strategy to prevent new infections. We showed previously that a single immunization with a SIV-based integrase-defective lentiviral vector (IDLV) expressing the 1086.C HIV-1-envelope induced durable, high-magnitude immune responses in non-human primates (NHPs). In this study, we have further characterized the humoral responses by assessing antibody affinity maturation and antigen-specific memory B-cell persistence in two vaccinated macaques. These animals were also boosted with IDLV expressing the heterologous 1176.C HIV-1-Env to determine if neutralization breadth could be increased, followed by evaluation of the injection sites to assess IDLV persistence. IDLV-Env immunization was associated with persistence of the vector DNA for up to 6 months post immunization and affinity maturation of antigen-specific memory B cells.

引用

页数：13

共 64 条

[1] Antigenicity and Immunogenicity of RV144 Vaccine AIDSVAX Clade E Envelope Immunogen Is Enhanced by a gp120 N-Terminal Deletion [J].

Alam, S. Munir ;

Liao, Hua-Xin ;

Tomaras, Georgia D. ;

Bonsignori, Mattia ;

Tsao, Chun-Yen ;

Hwang, Kwan-Ki ;

Chen, Haiyan ;

Lloyd, Krissey E. ;

Bowman, Cindy ;

Sutherland, Laura ;

Jeffries, Thomas L., Jr. ;

Kozink, Daniel M. ;

Stewart, Shelley ;

Anasti, Kara ;

Jaeger, Frederick H. ;

Parks, Robert ;

Yates, Nicole L. ;

Overman, R. Glenn ;

Sinangil, Faruk ;

Berman, Phillip W. ;

Pitisuttithum, Punnee ;

Kaewkungwal, Jaranit ;

Nitayaphan, Sorachai ;

Karasavva, Nicos ;

Rerks-Ngarm, Supachai ;

Kim, Jerome H. ;

Michael, Nelson L. ;

Zolla-Pazner, Susan ;

Santra, Sampa ;

Letvin, Norman L. ;

Harrison, Stephen C. ;

Haynes, Barton F. .

JOURNAL OF VIROLOGY, 2013, 87 (03) :1554-1568

[2] Role of HIV membrane in neutralization by two broadly neutralizing antibodies [J].

Alam, S. Munir ;

Morelli, Marco ;

Dennison, S. Moses ;

Liao, Hua-Xin ;

Zhang, Ruijun ;

Xia, Shi-Mao ;

Rits-Volloch, Sophia ;

Sun, Li ;

Harrison, Stephen C. ;

Haynes, Barton F. ;

Chen, Bing .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (48) :20234-20239

[3] Stable gene transfer to muscle using non-integrating lentiviral vectors [J].

Apolonia, Luis ;

Waddington, Simon N. ;

Fernandes, Carolina ;

Ward, Natalie J. ;

Bouma, Gerben ;

Blundell, Michael P. ;

Thrasher, Adrian J. ;

Collins, Mary K. ;

Philpott, Nicola J. .

MOLECULAR THERAPY, 2007, 15 (11) :1947-1954

[4] Novel vaccine vectors for HIV-1 [J].

Barouch, Dan H. ;

Picker, Louis J. .

NATURE REVIEWS MICROBIOLOGY, 2014, 12 (11) :765-771

[5] Lentiviral Vector-Based Prime/Boost Vaccination against AIDS: Pilot Study Shows Protection against Simian Immunodeficiency Virus SIVmac251 Challenge in Macaques [J].

Beignon, Anne-Sophie ;

Mollier, Karine ;

Liard, Christelle ;

Coutant, Frederic ;

Munier, Sandie ;

Riviere, Julie ;

Souque, Philippe ;

Charneau, Pierre .

JOURNAL OF VIROLOGY, 2009, 83 (21) :10963-10974

[6] A simple, versatile and efficient method to genetically modify human monocyte-derived dendritic cells with HIV-1-derived lentiviral vectors [J].

Berger, Gregory ;

Durand, Stephanie ;

Goujon, Caroline ;

Xuan-Nhi Nguyen ;

Cordeil, Stephanie ;

Darlix, Jean-Luc ;

Cimarelli, Andrea .

NATURE PROTOCOLS, 2011, 6 (06) :806-816

[7]

Blasi M, 2018, COMMUN BIOL, DOI [10.6084/m9.figshare.6849011.v1, DOI 10.6084/M9.FIGSHARE.6849011.V1]

[8] Antibody-virus co-evolution in HIV infection: paths for HIV vaccine development [J].

Bonsignori, Mattia ;

Liao, Hua-Xin ;

Gao, Feng ;

Williams, Wilton B. ;

Alam, S. Munir ;

Montefiori, David C. ;

Haynes, Barton F. .

IMMUNOLOGICAL REVIEWS, 2017, 275 (01) :145-160

[9] Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors [J].

Bonsignori, Mattia ;

Hwang, Kwan-Ki ;

Chen, Xi ;

Tsao, Chun-Yen ;

Morris, Lynn ;

Gray, Elin ;

Marshall, Dawn J. ;

Crump, John A. ;

Kapiga, Saidi H. ;

Sam, Noel E. ;

Sinangil, Faruk ;

Pancera, Marie ;

Yang Yongping ;

Zhang, Baoshan ;

Zhu, Jiang ;

Kwong, Peter D. ;

O'Dell, Sijy ;

Mascola, John R. ;

Wu, Lan ;

Nabel, Gary J. ;

Phogat, Sanjay ;

Seaman, Michael S. ;

Whitesides, John F. ;

Moody, M. Anthony ;

Kelsoe, Garnett ;

Yang, Xinzhen ;

Sodroski, Joseph ;

Shaw, George M. ;

Montefiori, David C. ;

Kepler, Thomas B. ;

Tomaras, Georgia D. ;

Alam, S. Munir ;

Liao, Hua-Xin ;

Haynes, Barton F. .

JOURNAL OF VIROLOGY, 2011, 85 (19) :9998-10009

[10] Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial [J].

Buchbinder, Susan P. ;

Mehrotra, Devon V. ;

Duerr, Ann ;

Fitzgerald, Daniel W. ;

Mogg, Robin ;

Li, David ;

Gilbert, Peter B. ;

Lama, Javier R. ;

Marmor, Michael ;

del Rio, Carlos ;

McElrath, M. Juliana ;

Casimiro, Danilo R. ;

Gottesdiener, Keith M. ;

Chodakewitz, Jeffrey A. ;

Corey, Lawrence ;

Robertson, Michael N. .

LANCET, 2008, 372 (9653) :1881-1893

← 1 2 3 4 5 6 7 →