Electrospun Nanodiamond-Silk Fibroin Membranes: A Multifunctional Platform for Biosensing and Wound-Healing Applications

被引：69

作者：

Khalid, Asma ^{[1
,2
]}

Bai, Dongbi ^{[1
]}

Abraham, Amanda N. ^{[1
,2
]}

Jadhav, Amit ^{[3
]}

Linklater, Denver ^{[1
]}

Matusica, Alex ^{[4
]}

Duy Nguyen ^{[1
]}

Murdoch, Billy James ^{[1
]}

Zakhartchouk, Nadia ^{[1
]}

Dekiwadia, Chaitali ^{[1
]}

Reineck, Philipp ^{[1
,2
]}

Simpson, David ^{[5
]}

Vidanapathirana, Achini K. ^{[6
,7
]}

Houshyar, Shadi ^{[8
]}

Bursill, Christina A. ^{[6
,7
]}

Ivanova, Elena P. ^{[1
]}

Gibson, Brant C. ^{[1
,2
]}

机构：

[1] RMIT Univ, Sch Sci, Melbourne, Vic 3001, Australia

[2] RMIT Univ, Australian Res Council ARC, Ctr Excellence Nanoscale BioPhoton CNBP, Sch Sci, Melbourne, Vic 3001, Australia

[3] RMIT Univ, Sch Fash & Text, Brunswick, Vic 3056, Australia

[4] Flinders Univ S Australia, Sch Comp Sci Engn & Math, Clovelly Pk, SA 5042, Australia

[5] Univ Melbourne, Sch Phys, Parkville, Vic 3052, Australia

[6] Univ Adelaide, Australian Res Council ARC, Ctr Excellence Nanoscale BioPhoton CNBP, Adelaide Med Sch,Fac Hlth & Med Sci, Adelaide, SA 5005, Australia

[7] South Australian Hlth & Med Res Inst SAHMRI, Vasc Res Ctr, Lifelong Hlth, Adelaide, SA 5001, Australia

[8] RMIT Univ, Sch Engn, Melbourne, Vic 3001, Australia

来源：

ACS APPLIED MATERIALS & INTERFACES | 2020年 / 12卷 / 43期

基金：

澳大利亚研究理事会;

关键词：

electrospinning; nitrogen vacancy center; nanodiamonds; silk fibroin; temperature sensing; biocompatible materials; wound healing; INFECTION; DIAMOND; FABRICATION; SENSORS; SPHERES; GLYCOL; FILMS; WATER;

D O I：

10.1021/acsami.0c15612

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Next generation wound care technology capable of diagnosing wound parameters, promoting healthy cell growth, and reducing pathogenic infections noninvasively would provide patients with an improved standard of care and accelerated wound repair. Temperature is one of the indicating biomarkers specific to chronic wounds. This work reports a hybrid, multifunctional optical material platform-nanodiamond (ND)-silk membranes as biopolymer dressings capable of temperature sensing and promoting wound healing. The hybrid structure was fabricated through electrospinning, and 3D submicron fibrous membranes with high porosity were formed. Silk fibers are capable of compensating for the lack of an extracellular matrix at the wound site, supporting the wound-healing process. Negatively charged nitrogen vacancy (NV-) color centers in NDs exhibit optically detected magnetic resonance (ODMR) and act as nanoscale thermometers. This can be exploited to sense temperature variations associated with the presence of infection or inflammation in a wound, without physically removing the dressing. Our results show that the presence of NDs in the hybrid ND-silk membranes improves the thermal stability of silk fibers. NV- color centers in NDs embedded in silk fibers exhibit well-retained fluorescence and ODMR. Using the NV- centers as fluorescent nanoscale thermometers, we achieved temperature sensing in 25-50 degrees C, including the biologically relevant temperature window, for cell-grown ND-silk membranes. An enhancement (similar to 1.5x on average) in the temperature sensitivity of the NV- centers was observed for the hybrid materials. The hybrid membranes were further tested in vivo in a murine wound-healing model and demonstrated biocompatibility and equivalent wound closure rates as the control wounds. Additionally, the hybrid ND-silk membranes exhibited selective antifouling and biocidal propensity toward Gram-negative Pseudomonas aeruginosa and Escherichia coli, while no effect was observed on Gram-positive Staphylococcus aureus.

引用

页码：48408 / 48419

页数：12

共 47 条

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