N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative effect of transforming growth factor beta in endothelial cells

被引：9

作者：

Abel, Britain ^{[1
]}

Willoughby, Cara ^{[1
]}

Jang, Sungchan ^{[1
]}

Cooper, Laura ^{[1
]}

Xie, Leike ^{[1
]}

Vo-Ransdell, Chi ^{[1
]}

Sowa, Grzegorz ^{[1
]}

机构：

[1] Univ Missouri, Dept Med Pharmacol & Physiol, Columbia, MO 65212 USA

来源：

FEBS LETTERS | 2012年 / 586卷 / 19期

关键词：

Caveolin-2; N-terminal tyrosine and serine phosphorylation; TGF-beta; Smad3; PAI-1; Endothelial cell proliferation; GENE FAMILY; PROTEIN; EXPRESSION; SERINE-23; COMPONENT; SEQUENCE; MUSCLE;

D O I：

10.1016/j.febslet.2012.07.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we show that tyrosine phosphorylation of caveolin-2 (Cav-2) negatively regulates the anti-proliferative function of transforming growth factor beta (TGF-beta) in endothelial cells. In contrast to wild-type-Cav-2, retroviral re-expression of Y19/27F-Cav-2 in Cav-2 knockout endothelial cells did not affect anti-proliferative effect of TGF-beta compared to empty vector. Conversely, although less effective than wild-type, re-expression of S23/36A-Cav-2 reduced the effect of TGF-beta compared to empty vector. This differential effect of tyrosine and serine phosphorylation mutants of Cav-2 correlated with TGF-beta-induced Smad3 phosphorylation and transcriptional activation of plasminogen activator inhibitor-1. Thus tyrosine-phosphorylated Cav-2 counteracts anti-proliferative effect of TGF-beta in endothelial cells. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3317 / 3323

页数：7

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