A nitroreductase and acidity detecting dual functional ratiometric fluorescent probe for selectively imaging tumor cell

被引：25

作者：

He, Zhaoshuai ^{[1
]}

Chou, Yajie ^{[1
]}

Zhou, Hanxin ^{[1
]}

Zhang, Han ^{[1
]}

Cheng, Tanyu ^{[1
]}

Liu, Guohua ^{[1
]}

机构：

[1] Shanghai Normal Univ, Dept Chem, Key Lab Resource Chem, Key Lab Rare Earth Funct Mat,Minist Educ, 100 Guilin Rd, Shanghai 200234, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2018年 / 16卷 / 17期

基金：

中国国家自然科学基金;

关键词：

IN-VIVO; FUNCTION-PH; HYPOXIA; CANCER; OXYGEN; PURIFICATION; NANOSENSORS; BIOMARKERS; DOTS;

D O I：

10.1039/c8ob00670a

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Nitroreductase and acidity are common features of a tumor, which is associated with serious disease with a high mortality rate. However, solo detection of nitroreductase or acidic environments may cause false-positive results. Thus, it is very meaningful to develop dual functional probes for detecting nitroreductase and acidity, as these could be used for accurate tumor imaging. Here we report on the first dual functional ratiometric fluorescent probe, NAFP, for acidity and nitroreductase detection. This probe enables the measurement of the acidic microenvironment and the concentration of nitroreductase at the same time and the same site. The probe can obviously distinguish acidity, nitroreductase, and nitroreductase in an acidic environment. Furthermore, confocal fluorescence imaging of A549 cells indicates that NAFP can detect acidity and expressed nitroreductase in living cells.

引用

页码：3266 / 3272

页数：7

共 49 条

[1] THE BIOACTIVATION OF 5-(AZIRIDIN-1-YL)-2,4-DINITROBENZAMIDE (CB1954) .1. PURIFICATION AND PROPERTIES OF A NITROREDUCTASE ENZYME FROM ESCHERICHIA-COLI - A POTENTIAL ENZYME FOR ANTIBODY-DIRECTED ENZYME PRODRUG THERAPY (ADEPT) [J].