Differentiation of epidermal keratinocytes is dependent on glucosylceramide:ceramide processing

被引:45
作者
Amen, Nicole [1 ]
Mathow, Daniel [1 ]
Rabionet, Mariona [1 ,2 ]
Sandhoff, Roger [1 ,2 ]
Langbein, Lutz [2 ]
Gretz, Norbert [3 ]
Jaeckel, Carsten [4 ]
Groene, Hermann-Josef [1 ]
Jennemann, Richard [1 ]
机构
[1] German Canc Res Ctr, Div Cellular & Mol Pathol, D-69120 Heidelberg, Germany
[2] German Canc Res Ctr, D-69120 Heidelberg, Germany
[3] Heidelberg Univ, Med Res Ctr Mannheim, Mannheim, Germany
[4] LMU Univ Munich, Univ Hosp Munich, Munich, Germany
关键词
BETA-GLUCOCEREBROSIDASE ACTIVITY; ACYL SPHINGOSYL GLUCOSE; STRATUM-CORNEUM; BARRIER FUNCTION; SKIN BARRIER; PERMEABILITY BARRIER; ATOPIC-DERMATITIS; OMEGA-HYDROXYCERAMIDES; CORNIFIED ENVELOPE; MURINE EPIDERMIS;
D O I
10.1093/hmg/ddt264
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Skin barrier function is primarily assigned to the outer epidermal layer, the stratum corneum (SC), mainly composed of corneocytes and lipid-enriched extracellular matrix. Epidermal ceramides (Cers) are essential barrier lipids, containing ultra-long-chain (ULC) fatty acids (FAs) with a unique ?-hydroxy group, which is necessary for binding to corneocyte proteins. In the SC, Cers are believed to derive from glucosylated intermediates, namely glucosylceramides (GlcCers), as surmised from human Gauchers disease and related mouse models. Tamoxifen (TAM)-induced deletion of the endogenous GlcCer-synthesizing enzyme UDP-glucose:ceramide glucosyltransferase (UGCG) in keratin K14-positive cells resulted in epidermal GlcCer depletion. Although free extractable Cers were elevated in total epidermis and as well in SC, protein-bound Cers decreased significantly in Ugcg(f/fK14CreERT2) mice, indicating glucosylation to be required for regular Cer processing as well as arrangement and extrusion of lipid lamellae. The almost complete loss of protein-bound Cers led to a disruption of the water permeability barrier (WPB). UGCG-deficient mice developed an ichthyosis-like skin phenotype marked by impaired keratinocyte differentiation associated with delayed wound healing. Gene expression profiling of Ugcg-mutant skin revealed a subset of differentially expressed genes involved in lipid signaling and epidermal differentiation/proliferation, correlating to human skin diseases such as psoriasis and atopic dermatitis. Peroxisome proliferator-activated receptor beta/delta (PPAR/), a Cer-sensitive transcription factor was identified as potential mediator of the altered gene sets.
引用
收藏
页码:4164 / 4179
页数:16
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