The P2X7 receptor from Xenopus laevis:: Formation of a large pore in Xenopus oocytes

被引：28

作者：

Paukert, M ^{[1
]}

Hidayat, S ^{[1
]}

Gründer, S ^{[1
]}

机构：

[1] Dept Otolaryngol, Res Grp Sensory Physiol, D-72076 Tubingen, Germany

来源：

FEBS LETTERS | 2002年 / 513卷 / 2-3期

关键词：

P2; purinoceptor; ion channel; two-electrode voltage-clamp; ion selectivity;

D O I：

10.1016/S0014-5793(02)02324-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purinergic P2X(7) receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X(7) has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X(7) receptor (xP2X(7)). xP2X(7) is only about 50% identical to the mammalian homologues, shows a broad tissue expression pattern, and has the electrophysiological characteristics typical of a P2X(7) receptor: low agonist affinity (EC50 about 2.6 mM) and a non-desensitizing current. Moreover, expression of xP2X(7) in Xenopus oocytes is sufficient to induce the formation of a large pore, which is permeable to large cations such as NMDG(+). Identification of a non-mammalian P2X(7) receptor may help to identify functionally important parts of the protein. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：253 / 258

页数：6

共 20 条

[1] An ATP-activated channel is involved in mitogenic stimulation of human T lymphocytes [J].

Baricordi, OR ;

Ferrari, D ;

Melchiorri, L ;

Chiozzi, P ;

Hanau, S ;

Chiari, E ;

Rubini, M ;

DiVirgilio, F .

BLOOD, 1996, 87 (02) :682-690

[2] P2X receptors: An emerging channel family [J].

Buell, G ;

Collo, G ;

Rassendren, F .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1996, 8 (10) :2221-2228

[3] Cloning and functional characterisation of the mouse P2X7 receptor [J].

Chessell, IP ;

Simon, J ;

Hibell, AD ;

Michel, AD ;

Barnard, EA ;

Humphrey, PPA .

FEBS LETTERS, 1998, 439 (1-2) :26-30

[4] ATP INDUCES NUCLEOTIDE PERMEABILITY IN RAT MAST-CELLS [J].

COCKCROFT, S ;

GOMPERTS, BD .

NATURE, 1979, 279 (5713) :541-542

[5] Neuronal P2X7 receptors are targeted to presynaptic terminals in the central and peripheral nervous systems [J].

Deuchars, SA ;

Atkinson, L ;

Brooke, RE ;

Musa, H ;

Milligan, CJ ;

Batten, TFC ;

Buckley, NJ ;

Parson, SH ;

Deuchars, J .

JOURNAL OF NEUROSCIENCE, 2001, 21 (18) :7143-7152

[6] THE P2Z PURINOCEPTOR - AN INTRIGUING ROLE IN IMMUNITY, INFLAMMATION AND CELL-DEATH [J].

DIVIRGILIO, F .

IMMUNOLOGY TODAY, 1995, 16 (11) :524-528

[7] ATP, a partial agonist for the P2Z receptor of human lymphocytes [J].

Gargett, CE ;

Cornish, JE ;

Wiley, JS .

BRITISH JOURNAL OF PHARMACOLOGY, 1997, 122 (05) :911-917

[8] Isoquinolines as antagonists of the P2X7 nucleotide receptor:: High selectivity for the human versus rat receptor homologues [J].

Humphreys, BD ;

Virginio, C ;

Surprenant, A ;

Rice, J ;

Dubyak, GR .

MOLECULAR PHARMACOLOGY, 1998, 54 (01) :22-32

[9] Neuronal P2X transmitter-gated cation channels change their ion selectivity in seconds [J].

Khakh, BS ;

Bao, XR ;

Labarca, C ;

Lester, HA .

NATURE NEUROSCIENCE, 1999, 2 (04) :322-330

[10] Functional evidence of distinct ATP activation sites at the human P2X7 receptor [J].

Klapperstück, M ;

Büttner, C ;

Schmalzing, G ;

Markwardt, F .

JOURNAL OF PHYSIOLOGY-LONDON, 2001, 534 (01) :25-35

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