Molecular typing of isolates obtained from aborted foetuses in Brucella-free Holstein dairy cattle herd after immunisation with Brucella abortus RB51 vaccine in Egypt

被引：13
作者：
Wareth, Gamal [1 ,2 ]
Melzer, Falk [1 ]
Boettcher, Denny [3 ]
El-Diasty, Mohamed [4 ]
El-Beskawy, Mohamed [5 ]
Rasheed, Nesma [4 ]
Schmoock, Gernot [1 ]
Roesler, Uwe [6 ]
Sprague, Lisa D. [1 ]
Neubauer, Heinrich [1 ]
机构：
[1] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Bacterial Infect & Zoonoses, Naumburger Str 96a, D-07743 Jena, Germany
[2] Benha Univ, Fac Vet Med, Moshtohor 13736, Toukh, Egypt
[3] Univ Leipzig, Fac Vet Med, Inst Pathol, Tierklin 33, D-04103 Leipzig, Germany
[4] Anim Hlth Res Inst, Mansoura Prov Lab, Mansoura, Egypt
[5] Mansoura Univ, Fac Vet Med, Mansoura, Egypt
[6] Free Univ Berlin, Inst Anim Hyg & Environm Hlth, Robert von Ostertag Str 7-13, D-14163 Berlin, Germany
来源：
ACTA TROPICA | 2016年 / 164卷
关键词：
Brucella abortus; Cattle; MLVA; RB51; Abortion; Egypt; STRAIN RB51; ANIMAL BRUCELLOSIS; MELITENSIS; INFECTION; CALVES; ASSAY; OVIS; PCR;
D O I：
10.1016/j.actatropica.2016.09.019
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Bovine brucellosis is endemic in Egypt in spite of application of surveillance and control measures. An increase of abortions was reported in a Holstein dairy cattle herd with 600 animals in Damietta governorate in Egypt after immunisation with Brucella (B.) abortus RB51 vaccine. Twenty one (10.6%) of 197 vaccinated cows aborted after 3 months. All aborted cows had been tested seronegative for brucellosis in the past 3 years. B. abortus was isolated from four foetuses. Conventional biochemical and bacteriological identification and polymerase chain reaction (PCR) confirmed two B. abortus biovar (bv.) 1 smooth and two B. abortus rough strains. None of the B. abortus isolates were identified as RB51. Genotyping analysis by multiple locus of variable number tandem repeats analysis based on 16 markers (MLVA-16) revealed two different profiles with low genetic diversity. B. abortus bv1 was introduced in the herd and caused abortions. (C) 2016 Elsevier B.V. All rights reserved.
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页码：267 / 271
页数：5
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