Efficient multi-enzymatic synthesis of D-xylulose 5-phosphate

被引：66

作者：

Zimmermann, FT

Schneider, A

Schörken, U

Sprenger, GA

Fessner, WD

机构：

[1] Rhein Westfal TH Aachen, Inst Organ Chem, D-52056 Aachen, Germany

[2] Forschungszentrum Julich, Inst Biotechnol 1, D-52425 Julich, Germany

来源：

TETRAHEDRON-ASYMMETRY | 1999年 / 10卷 / 09期

关键词：

D O I：

10.1016/S0957-4166(99)00166-4

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Pure D-threo-2-pentulose 5-phosphate, a valuable substrate required for enzymatic assays, is prepared in gram-quantities (82% overall yield) by an enzymatic one-pot procedure starting from the readily available precursors hydroxypyruvate and fructose 1,6-bisphosphate. The procedure is based on a stereospecific C-C bond formation catalyzed by the recombinant transketolase A from Escherichia coli. (C) 1999 Elsevier Science Ltd. All rights reserved.

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页码：1643 / 1646

页数：4

相关论文

共 23 条

[1] Continuous production of erythrulose using transketolase in a membrane reactor [J].

Bongs, J ;

Hahn, D ;

Schorken, U ;

Sprenger, GA ;

Kragl, U ;

Wandrey, C .

BIOTECHNOLOGY LETTERS, 1997, 19 (03) :213-215

[2]

de la HABA G., 1955, JOUR BIOL CHEM, V214, P409

[3] PREPARATION AND PROPERTIES OF LITHIUM HYDROXYPYRUVATE AND HYDROXYPYRUVIC ACID [J].

DICKENS, F ;

WILLIAMSON, DH .

BIOCHEMICAL JOURNAL, 1958, 68 :74-81

[4] Enzyme mediated C-C bond formation [J].

Fessner, WD .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1998, 2 (01) :85-97

[5]

FESSNER WD, 1996, TOP CURR CHEM, V184, P97

[6] IMPROVED PRODUCTION AND STABILITY OF ESCHERICHIA-COLI RECOMBINANTS EXPRESSING TRANSKETOLASE FOR LARGE-SCALE BIOTRANSFORMATION [J].

FRENCH, C ;

WARD, JM .

BIOTECHNOLOGY LETTERS, 1995, 17 (03) :247-252

[7] Recent advances in the chemoenzymatic synthesis of carbohydrates and carbohydrate mimetics [J].

Gijsen, HJM ;

Qiao, L ;

Fitz, W ;

Wong, CH .

CHEMICAL REVIEWS, 1996, 96 (01) :443-473

[8]

Hobbs GR, 1996, J BIOTECHNOL, V45, P173

[9] ENZYME-CATALYZED CARBON CARBON BOND FORMATION - USE OF TRANSKETOLASE FROM ESCHERICHIA-COLI [J].

HOBBS, GR ;

LILLY, MD ;

TURNER, NJ ;

WARD, JM ;

WILLETS, AJ ;

WOODLEY, JM .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1993, (02) :165-166

[10] A mechanism for fatty acid inhibition of glucose utilization in liver - Role of xylulose 5-P [J].

Liu, YQ ;

Uyeda, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (15) :8824-8830