Immunolocalization of hyaluronic acid and inter-alpha-trypsin inhibitor in mice

被引:20
作者
Kobayashi, H [1 ]
Sun, GW [1 ]
Terao, T [1 ]
机构
[1] Hamamatsu Univ Sch Med, Dept Obstet & Gynecol, Hamamatsu, Shizuoka 4313192, Japan
关键词
hyaluronic acid; immunohistochemistry; inter-alpha-trypsin inhibitor; localization; mouse (CD-1);
D O I
10.1007/s004410051320
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The direct interaction of hyaluronic acid (HA) and heavy chain (HC) of the inter-alpha-trypsin inhibitor (I alpha I) family plays a critical role in the organization and stabilization of the extracellular matrix. The aim of the present investigation was to elucidate the distribution of the I alpha I HC and HA in adult mouse tissues. An immunohistochemical method using a rabbit polyclonal antibody raised against mouse I alpha I heavy-chain peptide and a specific probe for HA (biotinylated HA-binding protein) was used to demonstrate an immunolocalization of I alpha I HC and HA. Distribution and localization of HA was of three types, namely, colocalization with I alpha I HC itself (cartilaginous tissue and ovary), localization around I alpha I HC immunostaining (lung, intestine and skeletal muscle), and localization at a small distance from I alpha I HC or a different distribution pattern (brain, liver, skin and kidney). These results indicate that I alpha I HC could function as an HA-rich matrix stabilizer on the cells of cartilage and maturing ovary, in which I alpha I HC shows colocalization with its predominant ligand, HA.
引用
收藏
页码:587 / 597
页数:11
相关论文
共 50 条
[41]   THERAPEUTIC EFFECTS OF HYALURONIC ACID IN PERITONITIS-INDUCED SEPSIS IN MICE [J].
Lee, Jae Hoon ;
Liu, Airan ;
Park, Jeong-Hyun ;
Kato, Hideya ;
Hao, Qi ;
Zhang, Xiwen ;
Zhou, Li ;
Lee, Jae-Woo .
SHOCK, 2020, 54 (04) :488-497
[42]   Adhesion preventive effect of hyaluronic acid after intraperitoneal surgery in mice [J].
Sawada, T ;
Hasegawa, K ;
Tsukada, K ;
Kawakami, S .
HUMAN REPRODUCTION, 1999, 14 (06) :1470-1472
[43]   Ontogeny of inter-alpha inhibitor protein (IAIP) expression in human brain [J].
Kim, Boram ;
De La Monte, Suzanne ;
Hovanesian, Virginia ;
Patra, Aparna ;
Chen, Xiaodi ;
Chen, Ray H. ;
Miller, Miles C. ;
Pinar, Mehmet Halit ;
Lim, Yow-Pin ;
Stopa, Edward G. ;
Stonestreet, Barbara S. .
JOURNAL OF NEUROSCIENCE RESEARCH, 2020, 98 (05) :869-887
[44]   Cross talk between COX-2 inhibitor and hyaluronic acid in osteoarthritic chondrocytes [J].
Takahashi, T ;
Uemura, Y ;
Taguchi, H ;
Ogawa, Y ;
Yoshida, S ;
Toda, M ;
Kobayashi, T ;
Seguchi, H ;
Tani, T .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2004, 14 (02) :139-144
[45]   Urinary trypsin inhibitor down-regulates hyaluronic acid fragment-induced prostanoid release in cultured human amnion cells by inhibiting cyclo-oxygenase-2 expression [J].
Kobayashi, H ;
Sun, GW ;
Terao, T .
MOLECULAR HUMAN REPRODUCTION, 1999, 5 (07) :662-667
[46]   DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice [J].
Ito, Tomoko ;
Yoshihara, Chieko ;
Hamada, Katsuyuki ;
Koyama, Yoshiyuki .
BIOMATERIALS, 2010, 31 (10) :2912-2918
[47]   ITIH5, a novel member of the inter-α-trypsin inhibitor heavy chain family is downregulated in breast cancer [J].
Himmelfarb, M ;
Klopocki, E ;
Grube, S ;
Staub, E ;
Klaman, I ;
Hinzmann, B ;
Kristiansen, G ;
Rosenthal, A ;
Dürst, M ;
Dahl, E .
CANCER LETTERS, 2004, 204 (01) :69-77
[48]   Alpha-2-macroglobulin and hyaluronic acid as fibromarkers in patients with chronic hepatitis C [J].
Pitekova, B. ;
Kupcova, V ;
Uhlikova, E. ;
Mojto, V ;
Turecky, L. .
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, 2017, 118 (11) :658-661
[49]   Fluorescent hyaluronic acid nanoprodrug: A tumor-activated autophagy inhibitor for synergistic cancer therapy [J].
Wu, Yundi ;
Liu, Xudong ;
Yao, Can ;
Chen, Jianqiang ;
Wu, Xilong ;
Zhu, Mingqiang .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 274
[50]   SYNTHETIC POLYSULFATED HYALURONIC-ACID IS A POTENT INHIBITOR FOR TUMOR-NECROSIS-FACTOR PRODUCTION [J].
CHANG, NS ;
INTRIERI, C ;
MATTISON, J ;
ARMAND, G .
JOURNAL OF LEUKOCYTE BIOLOGY, 1994, 55 (06) :778-784