Immunolocalization of hyaluronic acid and inter-alpha-trypsin inhibitor in mice

被引：20

作者：

Kobayashi, H ^{[1
]}

Sun, GW ^{[1
]}

Terao, T ^{[1
]}

机构：

[1] Hamamatsu Univ Sch Med, Dept Obstet & Gynecol, Hamamatsu, Shizuoka 4313192, Japan

来源：

CELL AND TISSUE RESEARCH | 1999年 / 296卷 / 03期

关键词：

hyaluronic acid; immunohistochemistry; inter-alpha-trypsin inhibitor; localization; mouse (CD-1);

D O I：

10.1007/s004410051320

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The direct interaction of hyaluronic acid (HA) and heavy chain (HC) of the inter-alpha-trypsin inhibitor (I alpha I) family plays a critical role in the organization and stabilization of the extracellular matrix. The aim of the present investigation was to elucidate the distribution of the I alpha I HC and HA in adult mouse tissues. An immunohistochemical method using a rabbit polyclonal antibody raised against mouse I alpha I heavy-chain peptide and a specific probe for HA (biotinylated HA-binding protein) was used to demonstrate an immunolocalization of I alpha I HC and HA. Distribution and localization of HA was of three types, namely, colocalization with I alpha I HC itself (cartilaginous tissue and ovary), localization around I alpha I HC immunostaining (lung, intestine and skeletal muscle), and localization at a small distance from I alpha I HC or a different distribution pattern (brain, liver, skin and kidney). These results indicate that I alpha I HC could function as an HA-rich matrix stabilizer on the cells of cartilage and maturing ovary, in which I alpha I HC shows colocalization with its predominant ligand, HA.

引用

页码：587 / 597

页数：11

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