Brief Research Report: Effect of Triiodothyronine on Hepatic Growth Hormone Receptor Expression in Primary Bovine Hepatocytes

被引：0

作者：

Stiensmeier, Vera ^{[1
]}

Schmicke, Marion ^{[1
,2
]}

机构：

[1] Martin Luther Univ Halle Wittenberg, Fac Nat Sci 3, Inst Agr & Nutr Sci, Anim Hlth Management, Halle, Germany

[2] Univ Vet Med Hannover, Clin Cattle Vet Endocrinol & Lab Diagnost, Hannover, Germany

来源：

FRONTIERS IN VETERINARY SCIENCE | 2022年 / 9卷

关键词：

hepatocytes; Cattle; growth hormone; growth hormone receptor; triiodothyronine; THYROID-HORMONES; GENE-EXPRESSION; MESSENGER-RNA; BLOOD-PLASMA; THYROXINE; PREGNANCY; LACTATION; LIVER; ACID; COWS;

D O I：

10.3389/fvets.2022.882102

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In previous studies, triiodothyronine (T3) was found to be lower in cows with ketosis and an effect of T3 on Growth Hormone Receptor (GHR) expression is described, e. g., in a human hepatoma cell line. Therefore, this study aimed to test whether T3 affects GHR messenger RNA (mRNA) expression in a well-established bovine hepatocyte model. Hepatocytes were kept in a sandwich culture and stimulated for 6 days with constant (10 mu g/ml) or decreasing (from 10 to 5 mu g/ml) T3 concentrations, and GHR, as well as IGF-1 mRNA expression, was measured using real-time polymerase chain reaction (RT-PCR). We could confirm in vitro that T3 has a stimulatory effect on GHR1A mRNA expression.

引用

页数：4

共 18 条

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