EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST)

被引:343
作者
Arendrup, Maiken C. [1 ]
Cuenca-Estrella, Manuel [2 ]
Lass-Floerl, Cornelia [3 ]
Hope, William [4 ]
机构
[1] Statens Serum Inst, Dept Microbiol Surveillance & Res, Unit Mycol, DK-2300 Copenhagen, Denmark
[2] Natl Ctr Microbiol, Inst Salud Carlos III, Mycol Reference Lab, Majadahonda, Spain
[3] Innsbruck Med Univ, Div Hyg & Microbiol, Innsbruck, Austria
[4] Univ Manchester, Univ Hosp S Manchester NHS Fdn Trust, Translat Res Facil Resp Med, NIHR,Manchester Acad Hlth Sci Ctr, Manchester, Lancs, England
关键词
antifungals; Candida; Cryptococcus; EUCAST; minimum inhibitory concentration; susceptibility testing; yeast; AMPHOTERICIN-B; SUSCEPTIBILITY;
D O I
10.1111/j.1469-0691.2012.03880.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Clin Microbiol Infect 2012; 18: E246E247 Abstract The European Committee on Antimicrobial Susceptibility Testing-Subcommittee on Antifungal Susceptibility Testing (EUCAST-AFST) has revised the EDef 7.1 document on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for fermentative yeasts. Changes are: dimethylsulphoxide is now the recommended solvent for caspofungin, micafungin and fluconazole; the shelf-life of plates containing the echinocandins prepared from stock solutions in dimethylsulphoxide is extended to 6 months at -80 degrees C; testing of amphotericin and Cryptococcus has been incorporated; and minimum inhibitory concentration ranges for quality control strains and anidulafungin are included.
引用
收藏
页码:E246 / E247
页数:2
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