Tissue microarray analysis of eIF4E and its downstream effector proteins in human breast cancer

被引：24

作者：

Kleiner, Heather E. ^{[1
]}

Krishnan, Prasad ^{[1
]}

Tubbs, Jesse ^{[2
]}

Smith, Mark ^{[2
]}

Meschonat, Carol ^{[2
]}

Shi, Runhua ^{[3
]}

Lowery-Nordberg, Mary ^{[4
]}

Adegboyega, Patrick ^{[4
]}

Unger, Marcia ^{[2
]}

Cardelli, James ^{[5
]}

Chu, Quyen ^{[2
]}

Mathis, Michael ^{[6
]}

Clifford, John ^{[7
]}

De Benedetti, Arrigo ^{[7
]}

Li, Benjamin D. L. ^{[2
]}

机构：

[1] Shreveport & LSUHSC Shreveport, Feist Weiller Canc Ctr, Breast Canc Focus Grp, Dept Pharmacol Toxicol & Neurosci, Shreveport, LA 71130 USA

[2] Shreveport & LSUHSC Shreveport, Feist Weiller Canc Ctr, Breast Canc Focus Grp, Dept Surg Oncol, Shreveport, LA 71130 USA

[3] Shreveport & LSUHSC Shreveport, Feist Weiller Canc Ctr, Breast Canc Focus Grp, Dept Med, Shreveport, LA 71130 USA

[4] Shreveport & LSUHSC Shreveport, Feist Weiller Canc Ctr, Breast Canc Focus Grp, Dept Pathol, Shreveport, LA 71130 USA

[5] Shreveport & LSUHSC Shreveport, Feist Weiller Canc Ctr, Breast Canc Focus Grp, Dept Microbiol & Immunol, Shreveport, LA 71130 USA

[6] Shreveport & LSUHSC Shreveport, Feist Weiller Canc Ctr, Breast Canc Focus Grp, Dept Cellular Biol & Anat, Shreveport, LA 71130 USA

[7] Shreveport & LSUHSC Shreveport, Feist Weiller Canc Ctr, Breast Canc Focus Grp, Dept Biochem & Mol Biol, Shreveport, LA 71130 USA

来源：

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH | 2009年 / 28卷

关键词：

INITIATION-FACTOR; 4E; CYCLIN D1; STAGE-I; OVEREXPRESSION; EXPRESSION; CARCINOMA; TRANSLATION; KINASE; EIF-4E; TLK1B;

D O I：

10.1186/1756-9966-28-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Eukaryotic initiation factor 4E (eIF4E) is elevated in many cancers and is a prognostic indicator in breast cancer. Many pro-tumorigenic proteins are selectively translated via eIF4E, including c-Myc, cyclin D1, ornithine decarboxylase (ODC), vascular endothelial growth factor (VEGF) and Tousled-like kinase 1B (TLK1B). However, western blot analysis of these factors in human breast cancer has been limited by the availability of fresh frozen tissue and the labor-intensive nature of the multiple assays required. Our goal was to validate whether formalin-fixed, paraffin-embedded tissues arranged in a tissue microarray (TMA) format would be more efficient than the use of fresh-frozen tissue and western blot to test multiple downstream gene products. Results: Breast tumor TMAs were stained immunohistochemically and quantitated using the ARIOL imaging system. In the TMAs, eIF4E levels correlated strongly with c-Myc, cyclin D1, TLK1B, VEGF, and ODC. Western blot comparisons of eIF4E vs. TLK1B were consistent with the immunohistochemical results. Consistent with our previous western blot results, eIF4E did not correlate with node status, ER, PR, or HER-2/neu. Conclusion: We conclude that the TMA technique yields similar results as the western blot technique and can be more efficient and thorough in the evaluation of several products downstream of eIF4E.

引用

页数：11

共 31 条

[1]

Anthony B, 1996, INT J CANCER, V65, P858, DOI 10.1002/(SICI)1097-0215(19960315)65:6<858::AID-IJC25>3.0.CO

[2]

2-Z

[3] High eIF4E, VEGF, and microvessel density in stage I to III breast cancer [J].