Effect of miR27a on Proliferation and Invasion in Colonic Cancer Cells

被引：14

作者：

Gao, Yang ^{[1
]}

Li, Bao-Dong ^{[1
]}

Liu, Yong-Gang ^{[1
]}

机构：

[1] Henan Canc Hosp, Dept Gen Surg, Zhengzhou, Henan, Peoples R China

来源：

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2013年 / 14卷 / 08期

关键词：

Colon cancer cells; miR27a; proliferation; invasion; PROGNOSTIC MICRORNAS; LUNG-CANCER; EXPRESSION; PROGRESSION; BIOMARKERS; MECHANISMS;

D O I：

10.7314/APJCP.2013.14.8.4675

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of this study was to detect the expression of miR196a, miR146a, miR27a and miR200a in patients with colon cancer, and investigate the effect of miR27a expression on proliferation and invasion in colonic cancer cells. RT-PCR was employed to detect the expression levels in colon cancers. Then, colon cancer cells were cultured and transfected with 100 nM of miR27a mimics (80 nmol/L) or 80 nM miR27a inhibitors (80 nmol/L) in 24-well plates. Proliferation and invasion of colonic cancer cells were then determined by CCK-8 and Transwell assays, respectively. Our data showed miR27a to be high-expressed in patients with colon cancer. In addition, proliferation and invasion in the miR27a mimic group were significantly higher than in the control group and negative group (P<0.05), while, proliferation and invasion in the miR27a inhibitor group were obviously lowered (P<0.05). In conclusion, high expression of miR27a may play an important role in enhancing proliferation and invasion of colon cancer cells.

引用

页码：4675 / 4678

页数：4

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