Anti-inflammatory and analgesic activities of the hydrolyzed sasanquasaponins from the defatted seeds of Camellia oleifera

被引:51
作者
Ye, Yong [1 ,2 ]
Xing, Haiting [1 ]
Chen, Xuelan [1 ]
机构
[1] S China Univ Technol, Sch Chem & Chem Engn, Guangzhou 510640, Peoples R China
[2] S China Univ Technol, Sch Chem & Chem Engn, Pharmaceut Engn Dept, Guangzhou 510640, Peoples R China
基金
中国国家自然科学基金;
关键词
Camellia oleifera; Anti-inflammatory; Analgesic activity; Sasanquasaponin; ANTIOXIDANT ACTIVITY; PROSTAGLANDIN E-2; INFLAMMATION; SAPONINS; FRACTION; ORIGIN; ROLES; PAIN;
D O I
10.1007/s12272-013-0138-y
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The defatted seeds of Camellia oleifera (Abel.) are used for stopping itching and pain in old days, the effective compounds need to be investigated. Sasanquasaponin as a rich fraction was extracted with 70 % ethanol, purified by AB-8 macro-reticular resin, crystallized in 80 % ethanol, and further hydrolyzed by 4 % hydroxyl potassium or 2 M hydrochloride. Anti-inflammatory activities of the extracts were measured by carrageenan-induced paw edema in rats and croton oil induced ear inflammation in mice; the analgesic activities were analyzed by hot plate test, acetic acid induced writhing in mice; the levels of pain mediators of IL-1 beta, TNF-alpha and PGE(2) were determined; the antioxidative activities in vivo were evaluated by MDA, SOD and GSH-Px in serum of rats. The extracts showed significant (p < 0.01) anti-inflammatory and analgesic activities, remarkably (p < 0.01) inhibited production of pro-inflammatory cytokines and PGE(2), decreased MDA and increased SOD and GSH-Px in serum. Inhibition of IL-1 beta, TNF-alpha and PGE(2) may contribute to their anti-inflammatory and analgesic effects; elimination of free radicals is also involved. The sapogenin and acid hydrolyzed product have better anti-inflammatory, analgesic effects, and stronger antioxidative activity than sasanquasaponin and alkaline hydrolyzed product, and they are better candidate medicines for inflammation and pain.
引用
收藏
页码:941 / 951
页数:11
相关论文
共 35 条
  • [1] CYTOKINE-MEDIATED INDUCTION OF CYCLO-OXYGENASE-2 BY ACTIVATION OF TYROSINE KINASE IN BOVINE ENDOTHELIAL-CELLS STIMULATED BY BACTERIAL LIPOPOLYSACCHARIDE
    AKARASEREENONT, P
    BAKHLE, YS
    THIEMERMANN, C
    VANE, JR
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1995, 115 (03) : 401 - 408
  • [2] Oral anti-inflammatory and anti-ulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnera ulmifolia (Turneraceae)
    Antonio, MA
    Brito, ARMS
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 1998, 61 (03) : 215 - 228
  • [3] ANTI-INFLAMMATORY ACTIVITY OF ORPANOXIN ADMINISTERED ORALLY AND TOPICALLY TO RODENTS
    BROOKS, RR
    BONK, KR
    DECKER, GE
    MILLER, KE
    [J]. AGENTS AND ACTIONS, 1985, 16 (05): : 369 - 376
  • [4] Foam Properties and Detergent Abilities of the Saponins from Camellia oleifera
    Chen, Yu-Fen
    Yang, Chao-Hsun
    Chang, Ming-Shiang
    Ciou, Yong-Ping
    Huang, Yu-Chun
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2010, 11 (11): : 4417 - 4425
  • [5] Crucial role of neutrophils in the development of mechanical inflammatory hypernociception
    Cunha, Thiago M.
    Verri, Waldiceu A., Jr.
    Schivo, Ieda R.
    Napimoga, Marcelo H.
    Parada, Carlos A.
    Poole, Stephen
    Teixeira, Mauro M.
    Ferreira, Sergio H.
    Cunha, Fernando Q.
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2008, 83 (04) : 824 - 832
  • [6] RELEASE OF PROSTAGLANDINS-E AND PROSTAGLANDINS-F IN AN ALGOGENIC REACTION AND ITS INHIBITION
    DERAEDT, R
    JOUQUEY, S
    DELEVALLEE, F
    FLAHAUT, M
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1980, 61 (01) : 17 - 24
  • [7] DIROSA M, 1972, J PHARM PHARMACOL, V24, P89, DOI 10.1111/j.2042-7158.1972.tb08940.x
  • [8] Fontenele JB, 1996, BRAZ J MED BIOL RES, V29, P643
  • [9] Relative hydrophobicity and lipophilicity of drugs measured by aqueous two-phase partitioning, octanol-buffer partitioning and HPLC. A simple model for predicting blood-brain distribution
    Gulyaeva, N
    Zaslavsky, A
    Lechner, P
    Chlenov, M
    McConnell, O
    Chait, A
    Kipnis, V
    Zaslavsky, B
    [J]. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2003, 38 (04) : 391 - 396
  • [10] Hou RuYan Hou RuYan, 2005, Journal of Anhui Agricultural University, V32, P369