Sulfated polysaccharide from the red algae Gelidiella acerosa: Anticoagulant, antiplatelet and antithrombotic effects

被引：45

作者：

Chagas, Francisco Diego da Silva ^{[1
]}

Lima, Glauber Cruz ^{[1
,2
]}

Santos, Valesca Ingrid Nobre dos ^{[1
]}

Costa, Luis Eduardo Castanheira ^{[1
]}

Sousa, Willer Malta de ^{[1
]}

Sombra, Venicios Goncalves ^{[3
]}

Araujo, Diego Freitas de ^{[4
]}

Barros, Francisco Clark Nogueira ^{[1
,5
]}

Marinho-Soriano, Eliane ^{[6
]}

Feitosa, Judith Pessoa de Andrade ^{[3
]}

Paula, Regina Celia Monteiro de ^{[3
]}

Pereira, Maria Goncalves ^{[4
]}

Freitas, Ana Lucia Ponte ^{[1
]}

机构：

[1] Univ Fed Ceara, Dept Biochem & Mol Biol, Lab Prot & Carbohydrates Marine Algae, BR-60455760 Fortaleza, CE, Brazil

[2] Univ Ctr Inta UNINTA, Campus Itapipoca, BR-62501040 Sobral, CE, Brazil

[3] Univ Fed Ceara, Dept Organ & Inorgan Chem, Lab Polymers, BR-60455760 Fortaleza, CE, Brazil

[4] Univ Estadual Ceara, Super Inst Biomed Sci, Lab Physiopharmacol Inflammat, BR-60714242 Fortaleza, CE, Brazil

[5] Univ Fed Rio Grande do Norte, Dept Oceanog & Limnol, Macroalgae Lab, BR-59014100 Natal, RN, Brazil

[6] Fed Inst Educ Sci & Technol Cearca, BR-63040540 Juazeiro Do Norte, CE, Brazil

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 159卷

关键词：

Sulfated polysaccharide; Chemical structure; Antithrombotic and antiplatelet activities; STRUCTURAL-CHARACTERIZATION; MARINE-ALGAE; FRACTION; AGAR; ANTIOXIDANT; CARRAGEENANS; SPECTROSCOPY; EXTRACTION; GALACTANS;

D O I：

10.1016/j.ijbiomac.2020.05.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sulfated polysaccharide from the red algae Gelidiella acerosa (GaSP) was obtained through enzymatic extraction and subjected to chemical characterization by HPSEC, elemental microanalysis, FT-IR and NMR spectroscopies. The GaSP anticoagulant activity was investigated through APTT and PT tests and platelet aggregation assessed by turbidimetry. The antithrombotic and hemorrhagic activities were evaluated by venous thrombosis and hemorrhagic tendency models, respectively. FT-IR and NMR demonstrated that GaSP is a sulfated agaran. HPSEC and elemental microanalysis revealed a peak molar mass of 284.8 kDa and a degree of sulfation of 0.63, respectively. This molecule prolonged the coagulation time in 2.1 times and inhibited the platelet aggregation by 45%. Furthermore, it showed significant dose-dependent antithrombotic effect of 40%, 64% and 80% at 0.1, 0.5 and 1 mg/kg, respectively, without hemorrhage. These results suggest that GaSP has promising antithrombotic. (c) 2020 Elsevier B.V. All rights reserved.

引用

页码：415 / 421

页数：7

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