COVID-19: molecular and serological detection methods

被引:26
作者
Dhamad, Ahmed E. [1 ,2 ]
Rhida, Muna A. Abdal [1 ,2 ]
机构
[1] Univ Arkansas, Cell & Mol Biol, Fayetteville, AR 72701 USA
[2] Wasit Univ, Dept Biol Sci, Kut, Wasit, Iraq
关键词
COVID-19; SARS-CoV-2; rRT-PCR; ID NOW COVID-19; Isothermal amplification; CRISPR-Cas12; LFA; ELISA; LATERAL FLOW ASSAY; CLINICAL CHARACTERISTICS; NEUTRALIZING ANTIBODIES; RAPID DETECTION; SARS-COV; SARS-COV-2; ELISA; 3,3',5,5'-TETRAMETHYLBENZIDINE; IMMUNOASSAY; PROTEIN;
D O I
10.7717/peerj.10180
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Since COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was declared as a pandemic disease by the World Health Organization in early 2020, many countries, organizations and companies have tried to find the best way to diagnose the virus and contain its spreading. SARS-CoV-2 is a positive-sense single RNA (+ssRNA) coronavirus and mainly spreads through droplets, respiratory secretions, and direct contact. The early detection of the virus plays a central role in lowering COVID19 incidents and mortality rates. Thus, finding a simple, accurate, cheap and quick detection approach for SARS-CoV-2 at early stage of the viral infection is urgent and at high demand all around the world. The Food and Drug Administration and other health agencies have declared Emergency Use Authorization to develop diagnostic methods for COVID-19 and fulfill the demand. However, not all developed methods are appropriate and selecting a suitable method is challenging. Among all detection methods, rRT-PCR is the gold standard method. Unlike molecular methods, serological methods lack the ability of early detection with low accuracy. In this review, we summarized the current knowledge about COVID-19 detection methods aiming to highlight the advantages and disadvantages of molecular and serological methods.
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页数:18
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